RESUMEN
OBJECTIVE@#To perform a review and data analysis of the pediatric projects funded by National Natural Science Foundation of China from 2009 to 2018, and to investigate the changes in key support areas, research interest, and research hotspots in pediatrics.@*METHODS@#The database of National Natural Science Foundation of China was searched to screen out pediatric research projects in 2009-2018, and the changes in funding intensity and research direction were analyzed.@*RESULTS@#From 2009 to 2018, a total of 1 017 pediatric projects were funded by National Natural Science Foundation of China, with 485 (47.69%) General Projects, 426 (41.89%) Youth Fund Projects, 73 (7.18%) Regional Research Programs, 16 (1.57%) Key Programs, 6 (0.59%) Outstanding Youth Fund Projects, 7 (0.69%) Overseas Programs, and 4 (0.39%) other programs. There was a seven-fold increase in the total amount of subsidies, which increased from 8.42 million yuan in 2009 to 66.25 million yuan in 2018. The projects with the Primary Discipline Code of reproductive system/perinatology/neonatology, nervous system and mental illness, or circulatory system received the highest amount of fund.@*CONCLUSIONS@#The support of pediatric projects by National Natural Science Foundation of China continues to increase in the past ten years, and the main types of projects are General Projects and Youth Fund Projects. Neonatology, nervous system/mental illness, and circulatory diseases are the main directions of funded projects.
Asunto(s)
Adolescente , Niño , Humanos , China , Administración Financiera , Fundaciones , Disciplinas de las Ciencias Naturales , NeonatologíaRESUMEN
Human papillomavirus (HPV)-induced cervical cancer is the second most common cancer among women worldwide. Despite the encouraging development of the preventive vaccine for HPV, a vaccine for both prevention and therapy or pre-cancerous lesions remains in high priority. Thus far, most of the HPV therapeutic vaccines are focused on HPV E6 and E7 oncogene. However these vaccines could not completely eradicate the lesions. Recently, HPV E5, which is considered as an oncogene, is getting more and more attention. In this study, we predicted the epitopes of HPV16 E5 by bioinformatics as candidate peptide, then, evaluated the efficacy and chose an effective one to do the further test. To evaluate the effect of vaccine, rTC-1 (TC-1 cells infected by rAAV-HPV16E5) served as cell tumor model and rTC-1 loading mice as an ectopic tumor model. We prepared vaccine by muscle injection. The vaccine effects were determined by evaluating the function of tumor-specific T cells by cell proliferation assay and ELISPOT, calculating the tumor volume in mice and estimating the survival time of mice. Our in vitro and in vivo studies revealed that injection of E5 peptide+CpG resulted in strong cell-mediated immunity (CMI) and protected mice from tumor growth, meanwhile, prolonged the survival time after tumor cell loading. This study provides new insights into HPV16 E5 as a possible target on the therapeutic strategies about cervical cancer.
RESUMEN
Human papillomavirus (HPV)-induced cervical cancer is the second most common cancer among women worldwide. Despite the encouraging development of the preventive vaccine for HPV, a vaccine for both prevention and therapy or pre-cancerous lesions remains in high priority. Thus far, most of the HPV therapeutic vaccines are focused on HPV E6 and E7 oncogene. However these vaccines could not completely eradicate the lesions. Recently, HPV E5, which is considered as an oncogene, is getting more and more attention. In this study, we predicted the epitopes of HPV16 E5 by bioinformatics as candidate peptide, then, evaluated the efficacy and chose an effective one to do the further test. To evaluate the effect of vaccine, rTC-1 (TC-1 cells infected by rAAV-HPV16E5) served as cell tumor model and rTC-1 loading mice as an ectopic tumor model. We prepared vaccine by muscle injection. The vaccine effects were determined by evaluating the function of tumor-specific T cells by cell proliferation assay and ELISPOT, calculating the tumor volume in mice and estimating the survival time of mice. Our in vitro and in vivo studies revealed that injection of E5 peptide+CpG resulted in strong cell-mediated immunity (CMI) and protected mice from tumor growth, meanwhile, prolonged the survival time after tumor cell loading. This study provides new insights into HPV16 E5 as a possible target on the therapeutic strategies about cervical cancer.
Asunto(s)
Adulto , Anciano , Animales , Femenino , Humanos , Ratones , Persona de Mediana Edad , Secuencia de Aminoácidos , Vacunas contra el Cáncer , Alergia e Inmunología , Línea Celular , Línea Celular Tumoral , Dependovirus , Genética , Regulación Viral de la Expresión Génica , Alergia e Inmunología , Vectores Genéticos , Genética , Papillomavirus Humano 16 , Genética , Alergia e Inmunología , Ratones Endogámicos C57BL , Neoplasias Experimentales , Alergia e Inmunología , Virología , Proteínas Oncogénicas Virales , Genética , Alergia e Inmunología , Infecciones por Papillomavirus , Alergia e Inmunología , Virología , Vacunas contra Papillomavirus , Alergia e Inmunología , Análisis de Supervivencia , Linfocitos T , Alergia e Inmunología , Metabolismo , Carga Tumoral , Alergia e Inmunología , Neoplasias del Cuello Uterino , Alergia e Inmunología , Virología , Vacunas de Subunidad , Alergia e InmunologíaRESUMEN
Objective: To detect the expressions of PI3K, AKT, p-AKT, and Ki67 in cervical intraepithelial neoplasia (CIN) and cervical carcinoma and explore their relation with the occurrence and development of cervical diseases and the clinical significance. Methods: The expression of PI3K, AKT, p-AKT, and Ki67 in cervical intraepithelial neoplasia (CIN) and cervical carcinoma were detected by immunohistochemical SP methods. Results: (1) The expressions of PI3K, AKT, p-AKT, and Ki67 protein were significantly higher in cervical carcinoma than CIN and normal cervical tissues. The difference was significant (P <0.05). There was no significant difference between adenocarcima and squamous adenocarcinoma. CIN tissues had higher expressions of PI3K, AKT, p-AKT, and Ki67 proteins than normal control (P <0.05) and the difference was increased with the elevated grade of CIN. (2) The expressions of PI3K, AKT, and p-AKT correlated with Ki67 in CIN tissues at various grades. (3) The expressions of PI3K and p-AKT had correlation with histological differentiation and clinical stage, but not with age, histological subtype, lymph node metastases. Expression of Ki67 was not associated with clinicopathological parameters. Conclusion: The aberrant expressions of PI3K, AKT, p-AKT, and Ki67 were involved in the occurrence and development of cervical cancer and related with the enhanced proliferation of tumor cells.