Expression and Clinical Significances of HGFA, Matriptase, HAI-1 and HAI-2 in Acute Myeloid Leukemia / 中国实验血液学杂志

<p><b>OBJECTIVE</b>To investigate the expression and clinical significances of HGFA, Matriptase, HAI-1 and HAI-2 in patients with acute myeloid leukemia (AML).</p><p><b>METHODS</b>The bone marrow samples from 91 AML patients, 41 AML patients in complete remission, and 32 normal controls were collected. Real time fluorescence quantitative RT-PCR (qRT-PCR) was used to detect the mRNA expressions levels of HGFA, Matriptase, HAI-1, HAI-2 . The expressions of these genes were compared among AML untreated group, the complete remission group and the healthy control group. The correlation of their expression with clinical characteristics was analyzed.</p><p><b>RESULTS</b>The level of HGFA in the AML untreated group was higher than that in the healthy control group（P<0.05）, while the HAI-2 mRNA level was lower than that in the healthy control group（P<0.05）. The mRNA levels of HAI-1 and Matriptase were not changed significantly in all groups. The HAI-2 mRNA expression level was significantly lower in the high white blood cell group (P<0.05).</p><p><b>CONCLUSION</b>The abnormal activation of HGF/c-Met signaling system in AML may result from the increase of HGFA expression and the decrease of HAI-2 expression of the upstream regulatory factors.</p>

Molecular Pathological Diagnosis of Mucosa-associated Lymphoid Tissue Lymphoma / 中国实验血液学杂志

<p><b>OBJECTIVE</b>To explore the molecular pathological diagnosis of mucosa-associated lymphoid tissue lymphoma(MALTL).</p><p><b>METHODS</b>Sixty MALTL paraffin embedding specimens were analyzed retrospectively. The distribution of the primary lesion, morphology, immunophenotype, IgH gene cloning rearrangement were evaluated.</p><p><b>RESULTS</b>The main risk area for the patients with MALTL was gastric area(37%), in the second place was salivary gland(20%), in the third place was intestine (12%), orbit and ocular adnexa(12%); at low magnification, MALTL specimens manifasted diffuse growth majority, a few of nodular structure, lymphoma cell forms were diversified; The results of immunohistochemical detection showed that the CD20 and the BCL-2 were positive, the CD3, CD5, CD10, CD23, cyclin D1 and CD21 were negative, 17 specimen kappa or lambda express more obviously, their sensibility was 28.33%(17/60); 61.67%(37/60) developed IgH gene rearrangement, 19 specimen IgH gene rearrangement monoclonal and kappa or lambda were negative, the positive rate of both combined detections was 68.33%.</p><p><b>CONCLUSION</b>The tissue morphologic characteristics and immuno-histochemistry detection are the basic means for MALTL diagnosis, the detection of IgH gene reasragement and Kappa or lamda restrictive expression has the practical importance for MALTL diagnosis, both combination can show higher positive rate for MALTL diagnosis.</p>

Development of a singleplex PCR assay targeting O-antigen modification genes for molecular serotyping of Shigella flexneri / 中华流行病学杂志

Objective To develop a singleplex PCR assay targeting O-antigen modification genes for molecular serotyping of Shigella (S.)flexneri.Methods Eight pairs of primer for O-antigen synthesis and modification genes of S.flexneriwere designed and used for developing an O-antigen modification gene-specific singleplex PCR assay to serotype 14 most common S.flexneri serotypes (1 a,1 b,1 c,2a,2b,3a,3b,4a,4b,5a,Y,X,Xv and F6).Bacterial pathogens which causing diarrheal disease were used for specificity detection.106 S.flexneri clinical isolates were serotyped by this method and compared with the slide agglutination method.Results An O-antigen modification,gene-specific singleplex PCR was developed.When six singleplex PCR reactions were performed,14 of the 15 recognized S.flexneri serotypes were identified,except for serotype Xv.The detection threshold ranged from 10 pg to 1 ng DNA in a 20 μ l reaction system.A high concordance between the singleplex PCR assay and slide agglutination were observed when 106 S.flexneri strains of various serotypes were analyzed with an exception that 1 serotype Y strain showed that it was carrying the additional defective gtr Ⅱ genes.Conclusion This method showed advantages over the traditional slide agglutination methods,and was promising when under application in the following situations as clinical diagnosis.

Chemical constituents from bark of Juglans mandshurica / 中草药

Objective To study the chemical constituents from the bark of Juglans mandshurica. Methods The compounds were isolated and purified by column chromatography on silica gel,HPLC,and recrystallization.Their structures were elucidated by the physicochemical and spectroscopic evidences. Results Fifteen compounds were identified as:4,8-dihydroxynaphthalenyl-O-?D-(6′-acetoxyl)gluco- pyranoside(Ⅰ),dihydrokaempferol(Ⅱ),juglone(Ⅲ),daucosterol(Ⅳ),kaempferol(Ⅴ),4,8-dihy- droxynaphthalenyl-1-O-?-D-[6′-O-(3″,5″-dimethoxy-4″-hydroxybenzoyl)] glucopyranoside(Ⅵ), kaempferol-3-O-?-L-rhamnoside(Ⅶ),3,3′-dimethoxylellagic acid(Ⅷ),naringenin(Ⅸ),quercetin (Ⅹ),reginolone(Ⅺ),quercetin-3-O-?-L-rhamnoside(Ⅻ),naringenin-7-O-?-D-glucoside(ⅩⅢ),4,8- dihydroxynaphthalenyl-1-O-?-glucoside(ⅩⅣ),4,5,8-trihydroxy-?-tetralone-5-O-?-D-[6′-O-(4″-hy- droxy-3″,5″-dimethoxy-benzoyl)] glucoside(ⅩⅤ).Conclusion CompoundⅠ(juglamanol)is a new compound.CompoundsⅡ,Ⅶ—Ⅸ,Ⅻ,andⅩⅢare isolated from plants of Carya Nutt.for the first time.