RESUMEN
The onset, severity, and ultimate outcome of malaria infection are influenced by parasite-expressed virulence factors as well as by individual host responses to these determinants. In both humans and mice, liver injury follows parasite entry, persisting to the erythrocytic stage in the case of infection with the fatal strain of Plasmodium falciparum. Hepatic nuclear factor (HNF)-1alpha is a master regulator of not only the liver damage and adaptive responses but also diverse metabolic functions. In this study, we analyzed the expression of host HNF-1alpha in relation to malaria infection and evaluated its interaction with the 5'-untranslated region of subtilisin-like protease 2 (subtilase, Sub2). Recombinant human HNF-1alpha expressed by a lentiviral vector (LV HNF-1alpha) was introduced into mice. Interestingly, differences in the activity of the 5'-untranslated region of the Pf-Sub2 promoter were detected in 293T cells, and LV HNF-1alpha was observed to influence promoter activity, suggesting that host HNF-1alpha interacts with the Sub2 gene.
Asunto(s)
Animales , Humanos , Ratones , Regiones no Traducidas 5'/genética , Línea Celular , ADN Protozoario/genética , Regulación de la Expresión Génica/genética , Vectores Genéticos , Factor Nuclear 1-alfa del Hepatocito/administración & dosificación , Interacciones Huésped-Parásitos , Inyecciones Intravenosas , Lentivirus/genética , Malaria Falciparum/metabolismo , Plasmodium falciparum/efectos de los fármacos , Regiones Promotoras Genéticas/genética , ARN Mensajero/genética , ARN Protozoario/genética , Proteínas Recombinantes , Transducción de Señal , Subtilisinas/genéticaRESUMEN
The onset, severity, and ultimate outcome of malaria infection are influenced by parasite-expressed virulence factors and individual host responses to these determinants. In both humans and mice, liver injury is involved after parasite entry, which persists until the erythrocyte stage after infection with the fatal strain Plasmodium falciparum (Pf). Hepatocyte growth factor (HGF) has strong anti-apoptotic effects in various kinds of cells, and also has diverse metabolic functions. In this work, Pf-subtilisin-like protease 2 (Pf-Sub2) 5'untranslated region (UTR) was analyzed and its transcriptional activity was estimated by luciferase expression. Fourteen TATA boxes were observed but only one Oct-1 and c-Myb were done. In addition, host HGF interaction with Pf-Sub2 was evaluated by co-transfection of HGF- and Pf-Sub2-cloned vector. Interestingly, -1,422/+12 UTR exhibited the strongest luciferase activity but -329 to +12 UTR did not exhibit luciferase activity. Moreover, as compared with the control of unexpressed HGF, the HGF protein suppressed luciferase expression driven by the 5'untranslated region of the Pf-Sub2 promoter. Taken together, it is suggested that HGF controls and interacts with the promoter region of the Pf-Sub2 gene.
Asunto(s)
Humanos , Regiones no Traducidas 5' , Fusión Artificial Génica , Línea Celular , Genes Reporteros , Factor de Crecimiento de Hepatocito/metabolismo , Hepatocitos/parasitología , Interacciones Huésped-Parásitos , Luciferasas/genética , Plasmodium falciparum/patogenicidad , Unión Proteica , Subtilisinas , Transcripción GenéticaRESUMEN
In this paper the background and advances of stem cell technique in stomatology were reviewed, especially the lately research of repair of maxillofacial defects with bone marrow stem cells, repair or reconstitution of teeth with dental pulp stem cells and repair of other tissues such as parotid with embryonic stem cell. Stem cell technique provides a new choice and extensive prospect of application for stomatology, therefore, deserves further research.