Application of improved shape-based interpolation algorithm in substantia nigra slices of rhesus monkeys / 中国组织工程研究

BACKGROUND:Studies have shown that pathological change of substantia nigra (SN) is the main reason for Parkinson's disease (PD).From the medical imaging,spatial location,size and 3D morphology analysis of the tiny SN structure are very important to PD's diagnosis and treatment evaluation.As the physiology of rhesus monkey is much similar to human's,it has been used as an ideal experiment subject in many scientific researches.OBJECTIVE:To modify traditional shape-based interpolation method for SN structure of the rhesus monkey brain slices.METHODS:To gain the middle layers between two slices,traditional shape-based interpolation used linear weighted mean algorithm.However,considering the impact of adjacent multi-layer to the image,the middle layers were obtained by non-linear weight.RESULTS AND CONCLUSION:The new interpolation algorithm was assessed with a favorable results.Also,this method was applied to the 3D reconstruction of SN structure.The results show that the new interpolation method can be used in nucleus group interpolation with sparse slices and tiny structures,and offer reference to the related disease research.

Phase contrast imaging and three-dimensional reconstruction of mouse liver under different conditions / 中国医疗器械杂志

The liver sample of mouse under different conditions is investigated in this paper, and the three-dimensional vessel system of mouse liver is established. The experiments were performed at X-ray imaging and biomedical application beamline (BL13W1) of Shanghai Synchrotron Radiation Facility (SSRF). The results show that dry liver sample has the best imaging quality. By the approach of filter back projection (FBP), the tomography images are obtained. The vessel tree is reconstructed by the use of surface rendering. It reveals that in-line phase contrast imaging technique has the ability to visualize structures inside mouse liver and has the potential to be used in liver imaging in the future.

Micron three-dimensional reconstruction image of a rabbit lower limb lymphedema model: Feasibility of synchrotron radiation in-line phase contrast imaging technique / 中国组织工程研究

BACKGROUND: The conventional X-ray imaging has low absorption contrast for lymphoedema, and the resolution is about mm level, which is not conducive to early diagnosis. Micro-imaging of biological soft tissues can be achieved by phase-contras imaging technique. OBJECTIVE: Using synchrotron radiation in-line phase contrast imaging technique to achieve the lymphoedema model imaging and observe the lesions characteristic of lymphatic vessels. DESIGN, TIME AND SETTING: Observational experiment. The lymphoedema model was prepared at the Capital Medical University from May to June in 2009. And the experiment was completed in Shanghai Synchrotron Radiation Facility (SSRF) BL13W1 in June 2009. MATERIALS: Five New Zealand rabbits were prepared for lower extremity lymphoedema models. METHODS: The lower extremity lymph node eradicates operation was performed on rabbits to result in lower extremity lymphoedema. And the synchrotron radiation in-line phase contrast imaging technology was used to collect the projected image of specimens within the 180°, and to reconstruct three-dimensional image. MAIN OUTCOME MEASURES: Severity of lymphatic vessels and the wall thickness. RESULTS: The lymphatic vessel was swollen, bent and distorted. And the lymphatic vessel generates lateral branches in order to promote lymphatic drainage, which was clearly observed from the image obtained by phase contrast imaging technology.CONCLUSION: The use of synchrotron radiation type coaxial imaging technology can be achieved for lymphedema model for micro-imaging, which has certain significance to the early diagnosis of lymphedema.

Segmentation of Hippocampus in Human Brain MRI Using Watersnakes / 대한의료정보학회지

OBJECTIVE: The algorithm of Watersnakes is used to segment the hippocampus on one slice of MR image. METHODS: This method integrates the watershed transform and the active contour algorithm. The watershed transform, based on mathematical morphology, is powerful and flexible for segmentation. However, it does not allow the characteristics of region boundaries to be included into the way that active contour algorithm do. So, oversegmentation is in the result, even to hippocampus. For watersnakes algorithm, the primitive contour of hippocampus can be obtained using watershed transform. Based on energy-driven, the contour of hippocampus develops into the ultimate result. In the process of energy-driven, the information relating to characteristics of region boundaries is involved. RESULTS: The results of watersnakes showed oversegmentation could be avoided, compared to the results of watershed transform. CONCLUSION: Watersnakes can use the advantage of watershed transform and active contour algorithm to improve the results of segmentation.