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Objective To investigate the effect of channel density on the gating properties of Anoctamin 1(Ano1,TMEM16A)Ca2+?activated chlo?ride channel. Methods Ano1 expression plasmids were transiently transfected into HEK293 cells. High density and low density of Ano1 was ob?tained after expressing the protein for 24 h and 6 h,respectively. Electrophysiological recordings were performed in the whole?cell patch clamp con?figuration. The activation kinetics of current traces was fitted by exponentials. Results The current density was significantly higher in cells express?ing Ano1 for 24 h than those expressing Ano1 for 6 h(P0.05). Conclusion Our findings suggested that chan?nel density regulates the gating of Ano1. High channel density promotes activation of Ano1.
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Objective To probe the curative effect of thiotepa and mitomycin C for the prevention of the relapse of pterygium after its operation.Methods One hundred and eighty-two cases with pterygium were randomly divided into two groups:one group of thiotepa(158 cases with 169 eyes)and the other group of mitomycin C (24 cases with 24 eyes) and a comparative study was conducted.Thiotepa was used through dropping on the third day after the operation(1∶2 000),while MMC was used in the same way during the operation and on the third day after the operation respectively(0.2g*L-1).Results In the group of thiotepa,the wounded skin on the cornea was repaired significantly more rapidly than that in the group of mitomycin C,and the rate of the relapse of pterygium was lower in the former group than that in the latter one.Conclusion Applying thiotepa by dropping after the excision of pterygium is a safe,simple and effective supplementary measure for the prevention of pterygium relapse and also effective for recurring cases.