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Rev. Inst. Med. Trop. Säo Paulo ; 45(3): 119-123, May-Jun. 2003.
Artículo en Inglés | LILACS | ID: lil-342162

RESUMEN

Salmonella Infantis has been the second most common serovar in Argentina in the last two years, being isolated mostly from paediatric hospitalised patients. In order to determine the clonal relationship among Salmonella Infantis strains, we examined 15 isolates from paediatric patient faeces in Argentina (12 geographically related and 3 geographically non-related) by using antimicrobial susceptibility, plasmid profiling, repetitive extragenic palindromic (REP) PCR, enterobacterial repetitive intergenic consensus (ERIC) PCR, and low-frequency restriction analysis of chromosomal DNA by pulsed field gel electrophoresis (PFGE). Four Spanish strains were included as controls of clonal diversity in molecular techniques. Antibiotype and plasmid profile was not useful as epidemiological tools. PFGE and REP-PCR were able to discriminate between Argentinean and Spanish isolates of Salmonella Infantis allowing to detect genetically related strains in three different cities. This finding indicates that a possible spread of a clone of this serovar in the North-eastern Region of Argentina has taken place in 1998


Asunto(s)
Humanos , Recién Nacido , Lactante , Preescolar , Técnicas de Tipificación Bacteriana , ADN Bacteriano , Salmonella enterica , Argentina , Dermatoglifia del ADN , ADN Bacteriano , Electroforesis en Gel de Campo Pulsado , Heces , Pruebas de Sensibilidad Microbiana , Plásmidos , Reacción en Cadena de la Polimerasa , Salmonella enterica , Infecciones por Salmonella
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