RESUMEN
We report the prevalence of human papillomavirus type 16 (HPV-16) variants in women with cervical lesions from the Federal District, Central Brazil. We analyzed 34 HPV-16 samples, identifying the sequence variations of E6 and L1 genes and correlating variant frequency with disease status. The most prevalent HPV-16 variant was the European (50 percent), followed by Asian-American (41.2 percent), African-1 (5.9 percent), and African-2 (2.9 percent). European and non-European variants appeared in equal frequencies among the cytological types of lesions - atypical squamous or glandular cells of undetermined significance, cytological alterations suggesting HPV infection, cervical intraepithelial neoplasias, squamous cell carcinoma, and adenocarcinoma.
Asunto(s)
Humanos , Femenino , Variación Genética , Papillomaviridae , Enfermedades del Cuello del Útero , Secuencia de Aminoácidos , Brasil , Datos de Secuencia Molecular , Papillomaviridae , Reacción en Cadena de la Polimerasa , Prevalencia , Índice de Severidad de la Enfermedad , Enfermedades del Cuello del ÚteroRESUMEN
As a contribution to the public health authorities in planning prophylactic and therapeutic vaccine strategies, we describe the prevalence of human papillomavirus (HPV) types in women presenting abnormal cytological results in Pap smear screening tests in the Federal District, Central Brazil. We studied 129 cervical scraping samples from women whose cytological tests showed either pre-neoplastic or neoplastic lesions. Amplification of HPV DNA was performed by polymerase chain reaction using consensus primers MY09 and MY11 followed by identification of isolates by restriction fragment length polymorphism. We detected HPV DNA in 62 percent of the samples, including HPV-16 in 43.8 percent, HPV-58 in 12.5 percent, HPV-31 in 10 percent, HPV-53 in 6.3 percent, each of HPV-18 and HPV-33 in 3.8 percent of the isolates. Other types (HPV-35, -52, -66, -CP8304, -6, -11, and -CP8061) were less frequent (= or < 2.5 percent each). The prevalence of HPV-58 was relatively higher in this population than in data in South America, but similar to results obtained in other studies in Latin America, Europe, and Eastern Asia. Case-control studies need to be carried out to establish the association between the prevalence of HPV types specially the less frequent high-risk types and cervical cancer.