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Braz. j. med. biol. res ; 24(1): 123-6, jan.-mar. 1991. ilus
Artículo en Inglés | LILACS | ID: lil-99591

RESUMEN

Multichannel experiments were carried out to investigate the Ca2+ - induced down-regulation of epithelial Na+ channels reconstituted into planar lipid bilayer membranes. Reconstitution was achueved by fusion of vesiculated apical membrane fragments to solvent-free planar lipid bilayers. We found that the presence of micromolar concentrations of Ca2+ on the side to which the vesicles were added substantially lowered the channel-mediated current. The inhibition was strongly influenced by pH. At pH 8.0 all the current was blocked by 1 mM calcium, whereas at pH 7.1 the inhibition was about 80%. The blocking kinetics was clearly voltage-dependent. The mechanism of blocking cannot be explained either in terms of interations with a single site, or by a model in which two blocking sites are assumed.


Asunto(s)
Animales , Amilorida/farmacología , Calcio/fisiología , Permeabilidad de la Membrana Celular , Sodio/metabolismo , Bufo marinus , Piel/metabolismo , Vejiga Urinaria/metabolismo
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