Missed detections of influenza A(H1)pdm09 by real-time RT–PCR assay due to haemagglutinin sequence mutation, December 2017 to March 2018, northern Viet Nam

Introduction@#There are two methods of reverse transcription polymerase chain reaction (RT–PCR) that have been the common methods to detect influenza infections: conventional and real-time RT–PCR. From December 2017 to March 2018, several missed diagnoses of influenza A(H1)pdm09 using real-time RT–PCR were reported in northern Viet Nam. This study investigated how these missed detections occurred to determine their effect on the surveillance of influenza.@*Methods@#The haemagglutinin (HA) segments of A(H1N1)pdm09 from both real-time RT-PCR positive and negative samples were isolated and sequenced. The primer and probe sets in the HA gene were checked for mismatches, and phylogenetic analyses were performed to determine the molecular epidemiology of these viruses.@*Results@#There were 86 positive influenza A samples; 32 were A(H1)pdm09 positive by conventional RT–PCR but were negative by real-time RT–PCR. Sequencing was conducted on 23 influenza (H1N1)pdm09 isolates that were recovered from positive samples. Eight of these were negative for A(H1)pdm09 by real-time RT–PCR. There were two different mismatches in the probe target sites of the HA gene sequences of all isolates (n = 23) with additional mismatches only at position 7 (template binding site) identified for all eight negative real-time RT–PCR isolates. The prime target sites had no mismatches. Phylogenetic analysis of the HA gene showed that both the positive and negative real-time RT–PCR isolates were grouped in clade 6B.1; however, the real-time RT–PCR negative viruses were located in a subgroup that referred to substitution I295V.@*Conclusion@#Constant monitoring of genetic changes in the circulating influenza A(H1N1)pdm09 viruses is important for maintaining the sensitivity of molecular detection assays.

Assessment on the effects by spinal hitting method to revover maternal breast milk source

Background: Recovery of maternal milk source is an urgent matter of many mothers in the breast feeding duration. Spinal hitting method of Nguyen Tham Tan has been applied on a lot of women lacked milk after giving birth but until now, there has not any resrearch on the method. Objectives: 1. To assess lactation effect resulting from spinal hitting method. 2. To evaluated effects of the method on milk quality and the concentration of prolactine in blood. Subjects and methods: 33 mothers who are lack milk after giving birth have been hit at points on their spine as follows: CS, C6, D2, L2-L3. These patients were treated during 15 days with one time a day, hitting duration of every time - 15 minutes. The study used open clinical method in comparison with the obtaining effects between pre and post reproducing. Results: Amount of breast milk per minute increases from 1.52ml \xb10.37ml (before treatment) to 2.20\xb10.42ml (after treatment). Number of children's feeding decreases and the concentrations of protein, lipid and glucose as well as vital elements in milk increase after treatment. The concentration of prolactine in blood increases from 2755.16m\xb5/ml (before treatment) to 3763.16m\xb5/ml (after treatment). Conclusion: Spinal hitting method has the effects of lactation, increase in prolactine concentration in bloods and positive effects on milk quality. \r\n", u'\r\n', u'\r\n', u'

The characteristics and value of spect myocardial perfusion imaging using dipyridamole in diagnosis of coronary artery disease

Background: Coronary artery disease (CAD) is the most common cardiovascular disease in developing countries. CAD also called coronary heart disease, is a condition in which plaque (plak) builds up inside the coronary arteries. These arteries supply your heart muscle with oxygen-rich blood. Objective: The purpose of the study was to investigate the characteristics and value of gated SPECT MPI with Technetium - 99m sestamibi using dipyridamole stress in diagnosis of CAD. Subjects and method: One hundred and forty - six suspected or known CAD patients, mean age 65.2 \xb1 9.11, underwent gated SPECT MPI with technetium - 99m sestamibi using dipyridamole stress. These patients was performed coronary angiography. Results: Of 146 patients, 33.6% had no adverse reaction to intravenous dipyridamole and no fatal adverse events (myocardial infarction, death ... ) was reported. The most common adverse reactions were headache (49.3%), chest pain (40.4%). Aminophyline was administrated to 25.7% of 146 patients and complete relief of symptoms in 91.3% of these patients. The sensitivity for detection of CAD was 95.2 % and the specificity and accuracy were 78.6% and 91.1 %. When excluded the patients with previous MI, these values were 88.9%; 80.0% and 85.7% respectively. Among the coronary branches, the sensitivity was highest in diagnosis of LAD and highest specificity was LCX lesions. Conclusions: The Technetium - 99m sestamibi gated SPECT using dipyridamole stress is a safety and valuable technique for the detection of CAD. \r\n', u' \r\n', u'

The feasibility of a school-based VI polysaccharide vaccine mass immunization campaign in Hue City, central Vietnam: streamlining a typhoid fever preventive strategy.

We report the coverage, safety, and logistics of a school-based typhoid fever immunization campaign that took place in Hue City, central Vietnam; a typhoid fever endemic area. A cluster-randomized evaluation-blinded controlled trial was designed where 68 schools (cluster) were randomly allocated the single dose Vi polysaccharide vaccine (Typherix) or the active control hepatitis A vaccine (Havrix). A safety surveillance system was implemented. A total of 32,267 children were immunized with a coverage of 57.5%. Strong predictors for vaccination were attending primary schools, peri-urban location of the school, and low family income. Human resources were mainly schoolteachers and the campaign was completed in about 1 month. Most adverse events reported were mild. Safe injection and safe sharp-waste disposal practices were followed. A typhoid fever school-based immunization campaign was safe and logistically possible. Coverage was moderate and can be interpreted as the minimum that could have been achievable because individual written informed consent procedures were sought for the first time in Hue City and the trial nature of the campaign. The lessons learned, together with cost-effectiveness results to be obtained by the end of follow-up period, will hopefully accelerate the introduction of Vi typhoid fever vaccine in Vietnam.

Results of study on the bioactivities of species of Kim That gynura procubens asteraceae

The extract of dried herb of Gynura procumberns, ratio 2:1 had an effect inhibiting symptoms of acute inflammation and is analgesic in experimentation. On the model inhibiting acute inflammatory ocdema, Gynura procum-bens extract inhibited 34.20% the inflammatory edema of mouse’s legs. On the model creating an acute pain by acetic acid 0.6% on white house mice, Gynura procumbens reduced the acute pain. On the model created a pain by temperature Gynura procumbens extract was also analgesic for about 29.05%.