Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 1 de 1
Filtrar
Añadir filtros








Intervalo de año
1.
J. appl. oral sci ; 27: e20180150, 2019. graf
Artículo en Inglés | LILACS, BBO | ID: biblio-975883

RESUMEN

Abstract Objectives This investigation aimed to assess the differentiation inhibitory effects of ProRoot MTA® (PMTA) and Biodentine® (BIOD) on osteoclasts originated from murine bone marrow macrophages (BMMs) and compare these effects with those of alendronate (ALD). Materials and Methods Mouse BMMs were cultured to differentiate into osteoclasts with macrophage colony-stimulating factor and receptor activator of NF-κB (RANKL), treated with lipopolysaccharide. After application with PMTA, BIOD, or ALD, cell toxicities were examined using WST-1 assay kit, and RANKL-induced osteoclast differentiation and activities were determined by resorption pit formation assay and tartrate-resistant acid phosphate (TRAP) staining. The mRNA levels of osteoclast activity-related genes were detected with quantitative real time polymerase chain reaction. Expressions of molecular signaling pathways were assessed by western blot. All data were statistically analyzed with one-way ANOVA and Tukey's post-hoc test (p<0.05). Results Mouse BMMs applied with PMTA, BIOD, or ALD showed highly reduced levels of TRAP-positive osteoclasts. The BIOD treated specimens suppressed mRNA expressions of cathepsin K, TRAP, and c-Fos. Nonetheless, it showed a lower effect than PMTA or ALD applications. Compared with ALD, PMTA and BIOD decreased RANKL-mediated phosphorylation of ERK1/2 and IκBα. Conclusions PMTA and BIOD showed the inhibitory effect on osteoclast differentiation and activities similar to that of ALD through IκB phosphorylation and suppression of ERK signaling pathways.


Asunto(s)
Animales , Ratones , Osteoclastos/efectos de los fármacos , Materiales de Obturación del Conducto Radicular/farmacología , Células de la Médula Ósea/efectos de los fármacos , Diferenciación Celular/efectos de los fármacos , Silicatos/farmacología , Compuestos de Calcio/farmacología , Alendronato/farmacología , Conservadores de la Densidad Ósea/farmacología , Osteoclastos/fisiología , Osteogénesis/efectos de los fármacos , Fosforilación/efectos de los fármacos , Resorción Radicular/prevención & control , Factores de Tiempo , Células de la Médula Ósea/citología , Supervivencia Celular/efectos de los fármacos , Células Cultivadas , Western Blotting , Reproducibilidad de los Resultados , Sistema de Señalización de MAP Quinasas/efectos de los fármacos , Proteínas I-kappa B/efectos de los fármacos , Ligando RANK/análisis , Ligando RANK/efectos de los fármacos , Reacción en Cadena en Tiempo Real de la Polimerasa , Fosfatasa Ácida Tartratorresistente
SELECCIÓN DE REFERENCIAS
DETALLE DE LA BÚSQUEDA