A method for staining the myentereric plexus

A method is presented for the rapid demonstration of the myenterifc plexus. A solution containing 30 per cent silver nitrate, 20 per cent potassium nitrate and 1 per cent glycine (50-50:1) followed by a solution of 1 per cent pyrogallol, ethyl alcohol (55 per cent) and a trace (0.002 per cent) of nitric acid were used on esophageal peels (Whole mounts) to stain fibers and neuron cell bodies. Neuronal perykaria and cell processes were visible. Since the nuclei of non neuronal supportinf cells were poorly stained and the muscle fibers has been dissected off, an image virtually free of background and visual interference was obtained