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Biol. Res ; 41(2): 205-215, 2008. ilus, graf
Artículo en Inglés | LILACS | ID: lil-495755

RESUMEN

Although several linker histone variants have been studied in both animal and plant organisms, little is known about their distribution during processes that involve alterations in chromatin function, such as differentiation, dedifferentiation and hormone treatment. In this study, we identified linker histone variants by using specific anti-histone Hl antibodies. Each variant's ratio to total Hl in the three developmental zones of maize (Zea mays L.) root and in callus cultures derived from them was estimated in order to define possible alterations either during plant cell differentiation or during their dedifferentiation. We also evaluated linker histone variants' ratios in the developmental zones of maize roots treated with auxin in order to examine the effects of exogenous applied auxin to linker histone variant distribution. Finally, immunohistochemical detection was used to identify the root tissues containing each variant and correlate them with the physiological status of the plant cells. According to the results presented in this study, linker histone variants' ratios are altered in the developmental zones of maize root, while they are similar to the meristematic zone in samples from callus cultures and to the differentiation zone in samples from roots treated with auxin. We propose that the alterations in linker histone variants' ratios are correlated with plant cell differentiation and dedifferentiation.


Asunto(s)
Histonas/análisis , Ácidos Indolacéticos/farmacología , Reguladores del Crecimiento de las Plantas/farmacología , Raíces de Plantas/química , Zea mays/química , Desdiferenciación Celular/efectos de los fármacos , Diferenciación Celular/efectos de los fármacos , Histonas/clasificación , Inmunohistoquímica , Raíces de Plantas/citología , Raíces de Plantas/efectos de los fármacos , Técnicas de Cultivo de Tejidos , Zea mays/citología , Zea mays/efectos de los fármacos
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