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Objective To introduce the performance of portable bedside CT (PBCT)-CereTom and evaluate its clinical value. Methods Three hundred and twenty severe or abasic patients from ICU of our hospital received PBCT-CereTom scanning of head. The performance of PBCT-CereTom and its initial clinical effect were analyzed. Results Three hundred and sixty three times of scanning with a slice of 5 mm were performed on these 320 patients, including 2 times in 43 and once in 277; these patients experienced 20-30 s for spiral condition and 50-60 s for non-spiral condition of scanning.Altogether,363 scanning got clear imaging without repeated examination for any patient,and no incidence and complication appeared.Conclusion PBCT-CereTom is safe and easy to operate, and can provide high-quality neuroimaging data,indicating that it is suitable for head examination of severe and abasic patients.
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<p><b>OBJECTIVE</b>To study the mechanism of multidrug resistance and its reversal by mdr-1 ribozyme in human ovarian cancer.</p><p><b>METHODS</b>The expression of mdr-1 and p-glycoprotein (p-gp) was studied by confocal laser microscope (Confocal), RT-PCR and Western blot analysis in adriamycin-resistant human ovarian cancer cell line (A2780/ADM) and adriamycin-sensitive one (A2780). The mdr-1 ribozyme was transfected into the A2780/ADM by Lipofectamine 2000 to overcome the multidrug resistance in ovarian cancer.</p><p><b>RESULTS</b>The expression of mdr-1 mRNA and p-gp in A2780/ADM was significantly higher than that in A2780. The expression of mdr-1 mRNA and p-gp in A2780/ADM was lowered after being transfected by mdr-1 ribozyme.</p><p><b>CONCLUSION</b>Multidrug resistance of A2780/ADM, possibly being caused by overexpression of mdr-1 gene, can be partially reversed by mdr-1 ribozyme.</p>