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Southeast Asian J Trop Med Public Health ; 1986 Mar; 17(1): 96-100
Artículo en Inglés | IMSEAR | ID: sea-34303

RESUMEN

Four immunological methods for diagnosis of giardiasis comprising complement fixation (CF) test, indirect hemagglutination (IHA) test, enzyme-linked immuno sorbent assay (ELISA) and lectin immuno test (LIT) were studied. Fifty sera from asymptomatic giardiasis patients, 40 from patients with other diseases and 50 from healthy controls were evaluated. The seropositive rates in asymptomatic giardiasis were 36% for CF, 58% for LIT, 30% for IHA and 72% for ELISA. The seropositive rates in patients with other diseases were 22.5% for CF, 52.5% for LIT, 12.5% for IHA and 67.5% for ELISA. The results suggest that the test of choice for giardiasis was CF with 88% specificity, nevertheless this test showed low sensitivity (36%). Other two tests, ELISA and LIT were more sensitive than CF with percent sensitivity of 72 and 58 respectively, but these two tests had severe disadvantages in being less specific with percentage specificity of 48 and 60 respectively.


Asunto(s)
Anticuerpos/análisis , Antígenos de Protozoos/inmunología , Pruebas de Fijación del Complemento , Concanavalina A , Ensayo de Inmunoadsorción Enzimática , Giardia/inmunología , Giardiasis/diagnóstico , Pruebas de Hemaglutinación/métodos , Humanos
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