Larvicidal activity of Dregea volubilis and Bombax malabaricum leaf extracts against the filarial vector Culex quinquefasciatus

OBJECTIVE@#To analyze the larvicidal activity of two plant leaf powder and leaf-extracts, Dregea volubilis and Bombax malabaricum against Culex quinquefasciatus.@*METHODS@#The larvicidal bioassay was done for powdered leaves of Dregea volubilis and Bombax malabaricum individually and their methanol extracts against first-, second-, third- and fourth-instar larval forms of Culex quinquefasciatus. Mortality rate was recorded after 24, 48 and 72 h of post-exposure. LC(50) and LC(90) values were calculated at different time intervals for third instar larvae.@*RESULTS@#All the graded concentration (0.1%, 0.2%, 0.3%, 0.4%, 0.5%) of powdered leaves showed significant (P <0.05) larval mortality. The mortality rate was higher in 50 ppm doses of methanolic extracts of both the plants against Culex quinquefaciatus. The corresponding LC(50) values were 56.97 ppm and 48.85 ppm, respectively after 24 h of exposure. There is no mortality of non-target organism such as Chironomous circumdatus, Oreochromis niloticus niloticus and Diplonichus annulatum within 72 h of post exposure to 0.1%, 0.2%, 0.3% crude powdered leaves and methanolic extracts of both the two individual plants under the laboratory condition. The results of preliminary qualitative phytochemical analysis of both the plants revealed the presence of many bioactive principles such as steroids, tannins, flavonoids, triterpenoids, saponins, etc. that may be responsible for their bio-control potentiality.@*CONCLUSIONS@#The results have shown potential and eco-friendly use of both plant extracts against larva of Culex quinquefasciatus for the first time.

Exploration of antioxidant efficacy of the methanol extract of leaves of coccinia grandis [L.] voigt. [family: cucurbitaceae] with some selective in vitro and ex vivo models

Coccinia grandis [L.] Voigt. [Family: Cucurbitaceae] is a climbing perennial herb distributed almost all over the world. The plant has ethnomedicinal significance to heal diabetes, inflammation, fever, pain, spasm and different infections. The intention of the present study is to explore the antioxidant activity of methanol extract of leaves of Coccinia grandis with the help of some selective in vitro and ex vivo models. The antioxidant activity was evaluated based on super oxide scavenging, RBC membrane stabilizing and inhibition of lipid peroxidation models. Super oxide scavenging activity was measured by in vitro assay methods employing both nitro blue tetrazolium chloride and alkaline dimethyl sulphoxide models. Inhibition of lipid peroxidation was measured by ex vivo assay method using FeSO4 and CCI4 induced lipid peroxidation models. Red Blood Cells membrane stabilization activity was measured on human blood RBC. The experimental data were compared with that of standard antioxidant like ascorbic acid and a-tocopherol acetate. All observed results indicated methanol extract of leaves of Coccinia grandis to possess antioxidant activity in a concentration dependant manner and very much comparable to that of selected standard drugs

Exploration of antioxidant efficacy of methanol extract of leaves of coccinia grandis [L.] voigt. [Family: cucurbitaceae] with some selective in vitro and ex vivo models

Coccinia grandis [L.] Voigt. [Family: Cucurbitaceae] is a climbing perennial herb distributed almost all over the world. The plant has ethnomedical significance to heal diabetes, inflammation, fever, pain, spasm and different infections. The intention of the study is to explore the antioxidant activity of methanol extract of leaves of Coccinia grandis with the help of some selective in vitro and ex vivo models. The antioxidant activity was evaluated based on super oxide scavenging, RBC membrane stabilizing and inhibition of lipid peroxidation models. Super oxide scavenging activity was measured by in vitro assay methods employing both nitro blue tetrazolium chloride and alkaline dimethyl sulphoxide models. Inhibition of lipid peroxidation was measured by ex vivo assay method using FeSO4 and CCI4 induced lipid peroxidation models. Red Blood Cells membrane stabilization activity was measured on human blood RBC. The experimental data were compared with that of standard antioxidant like ascorbic acid and alpha-tocopherol acetate. All observed results indicated methanol extract of the leaves of Coccinia grandis possesses antioxidant activity in a concentration dependant manner and very much comparable to that of selected standard drugs

Isolation and characterization of lupeol and beta-sitosterol from leaves of kigelia pinnata [bignoniaceae]

Triterpene and sterol components were isolated from unsaponifiable matter of petroleum ether extract of Kigelia pinnata leaves [family: Bignoniaceae] by column chromatography and were characterized by spectral and elemental analysis. These were identified as Lupeol and beta-sitosterol. These two components were first time reported from the leaf of this plant

Isolation and characterization of lupeol and p-sitosterol from leaves of kigelia pinnata [bignoniaceae]

Triterpene and sterol components were isolated from unsaponifiable matter of petroleum ether extract of Kigelia pinnata leaves, N.O. family Bignoniaceae by column chromatography and were characterized by spectral and elemental analysis. These were identified as Lupeol and beta-sitosterol These two components were first time reported from the leaf of this plant