Antimicrobial susceptibility test of microorganisms isolated from sputum culture of leukaemic patients with lower respiratory tract infections

The development of respiratory infection indicates either a defect in host defenses, exposure to a particularly virulent microorganism, or an ovenwhelming inoculum, as infectious agents gain entry to the lower respiratory tract through aspiration of upper airway resident flora. Better prognosis of patients with leukaemia over the last decade is at least partly due to the possibility of administering more intensive chemotherapy and to the successful introduction a wider array of antimicrobials. To evaluate the antimicrobial susceptibility of the isolates of both leukaemic and non-leukaemic patients with. LRTIs. The present study consisted of 50 adult leukaemic patients, 14 males and 36 females beside other 50 adult non-leukaemic patients, 25 males and 25 females were included, who were admitted to Baghdad Teaching Hospital, through the period from December 2003 through May 2004 with diagnosis of LRTIs. The antimicrobial susceptibility test was done upon the bacterial isolates according to Kirby-Bauer method. The most reliable antibiotics among leukaemic patients [acute myelogenous, acute lymphoblastic, chronic myeloid, chronic lymphocytic] according to antimicrobial susceptibility test, were in cosequence, ciprofloxacin, followed by cefotaxime, then gentamicin and equal influence by ceftriaxone, amikacin, cloxacillin followed by. trimethoprime-sulphamethoxazole, ampicillin, augmentin, finally by erythromycin. On the other spectrum, the most reliable antibiotics among non-leukaemic patients were in consequence ciprofloxacin, followed by trimethopritne-sulphamethoxazole, cefotaxime, an equal effect by ampicillin, gentamicin, and ceftriaxone, followed by augmentin, also an equal effect by cloxacillin and amikacin, finally by erythromycin. Antibiotic susceptibility test should be done for each bacterial isolate in order to prevent the development of progressive microbial resistance

Antibiotic sensitivity of pseudomonas stutzeri isolated from neurosurgical floor of specialized surgical hospital

The antibiotic sensitivity of forty isolates of Pseudomonas stutzeri from neurosurgical floor of the specialized surgical hospital was studied. The result indicated that twenty five isolates were sensitive to different antibiotics, six isolates were resistant to rifampicin [5mcg/disc], ampicillin [20mcg], garamycin [10mcg], cotrimoxazole [25mcg], nine isolates were resistant to garamycin and ceftriaxone [30mcg]. The objective of this study is to determine the antibiotic sensitivity of forty isolates of Pseudomonas stutzeri isolated from neurosurgical floor of the specialized surgical hospital. Pseudomonas stutzeri was identified according to Noble and Overman 1994. Antibiotic sensitivity using disc diffusion method according to Bauer and Turck 1966 was applied. Sixty and a half percent of Ps. stutzeri out the total forty isolates were sensitive to all antibiotics. Six isolates of Ps. stutzeri [15.0%] were resistant to rifampicin, ampicillin, garamycin and co-trimoxazole while nine [22.55%] isolates were resistant to garamycin and ceftriaxone. The present findings of in vitro study of antibiotic sensitivity test have shown that Ps. stutzeri are generally susceptible to aminoglycosides, carbapenems, monobactams, antipseudomonal penicillins, trimethoprime, sulfamethazole and third generation cephalosporin. The resistance of 6 isolates to rifampicin and 9 isolates to garamycin and ceftriaxone might draw attention of physicians to be aware to the treatment of Ps. stutzeri infections especially in the surgical ward and intensive care units