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Objective@#To explore the effect of bladder overdistention on uroflowmetry and post-void residual urine(PVR)in children with primary nocturnal enuresis (PNE).@*Methods@#A total of 136 children with PNE from March 2015 to June 2018 at Department of Pediatric Urodynamic Center, the First Affiliated Hospital of Zhengzhou University were enrolled, aged 5-14 years, with mean age of (7.96±2.48) years old.Uroflowmetry and PVR measurement were performed in the condition of over and no urinary distention, respectively.According to the initial urinary voided volume (VV), the children were divided into 3 groups, 25 mL≤ VV<50 mL(group 1), 50 mL≤VV<100 mL(group 2), and VV≥100 mL(group 3). The VV, maximum flow rate (Qmax), average flow rate (Qave), urine flow time, and PVR were compared interms of condition between the groups.Different types of flow curve were analyzed simultaneously.@*Results@#In the condition of bladder over distention, the VV, Qmax, Qave, urinary flow time and PVR of children were higher than those with no overdistention[group 1: (168.25±103.23) mL vs.(34.04±7.03) mL, (17.76±11.13)mL/s vs.(8.23±2.72) mL/s, (9.56±4.13) mL/s vs.(4.30±1.55) mL/s, (20.24±15.13) s vs.(8.64±4.27) s, (3.90±3.55) mL vs.(1.50±2.55) mL; group 2: (168.65±80.66) mL vs.(68.04±15.13) mL, (16.41±5.18) mL/s vs.(14.61±5.29) mL/s, (9.86±3.56) mL/s vs.(8.14±2.29) mL/s, (18.75±10.73) s vs.(9.41±3.47) s, (5.98±8.59) mL vs.(2.77±6.41) mL; group 3: (280.24±102.00) mL vs.(181.50±66.22) mL, (22.73±8.21) mL/s vs.(20.42±6.25) mL/s, (12.57±3.68) mL/s vs.(11.00±3.22) mL/s, (23.46±10.65) s vs.(17.01±6.90) s, (3.78±6.26) mL vs.(2.09±3.21) mL], and the differences were all significant(all P<0.05). Excluding physiological pseudomorphism, urinary flow curves of 114 children with initial urinary intention and excessive voiding were obtained.The incidence of Staccato urinary flow curve was significantly higher than that with no distention [6.14%(7/114 cases) vs.21.93%(25/114 cases), χ2=14.45, P<0.05].@*Conclusions@#Bladder overdistention induced significant influence on the uroflowmetry and PVR.
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AIM:To explore the expression and possible function of histamine H3 receptor (H3R) in striated myogenesis and the differentiated C2C12 cells.METHODS: H3R and myogenesis late marker myosin heavy chain (MHC) were detected at mRNA and protein levels during C2C12 myogenesis.H3R antagonist ciproxifan was added and the expression of the myogenesis early marker desmin, intermediate markers myogenin and MHC was detected.Differentia-ted myoblasts were loaded with Fluo-4 calcium indicator dye and the effect of R-( a)-methylhistamine ( RMeHA) on the cy-toplasmic calcium concentration was determined under the 200 mA electrical stimulation.RESULTS: The expression of H3R and MHC was increased during myogenesis.Ciproxifan incubation had no influence on the 3 striated myogenesis mar-kers (P>0.05).In C2C12 myoblasts, RMeHA (10 nmol/L~100 μmol/L) effectively diminished cytoplasmic calcium peak when the cells were electrically paced (P<0.05).The best inhibitory effect of RMeHA was observed at dose of 100 nM for 10 min and 20 min, which was higher than that for 5 min (P<0.05).CONCLUSION: H3R might have little effect on the myogenic differentiation, but diminishes cytoplasmic calcium peak of the differentiated myoblasts under electri-cal stimulation.
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Objective To investigate the voiding patterns of term and preterm newborns and whether voiding in term and preterm neonates was accompanied by any cortical arousal. Methods Between May 2013 and September 2013,64 hospitalized newborns at Neonatal Intensive Cave Unit in the Frist Affiliated Hospital of Zhengzhou University were recruited in this study. In these patients,31 cases were term newborns(20 male,11 female)and 33 cases were preterm newborns(19 male,14 female). The term and preterm newborns gestational ages at birth were(38. 2 ± 1. 2) weeks and(32. 1 ± 1. 6)weeks,weighted(3. 3 ± 0. 4)kg and(1. 7 ± 0. 3)kg,respectively and postnatal ages at study were[4 - 16(10. 5 ± 3. 6)]days and[4 - 16(11. 2 ± 3. 1)]days. The voiding volume(VV),post - void residual volumes(PRV),body movement rate and voiding frequency(VF)in 4 hours as well as the volume of milk and liquid fed at the same time frame were recorded and analyzed,retrospectively. At the same time electrocardiogram(ECG)and electroencephalogram(EEG)were recorded. The changes of heart rate(HR),EEG frequency,respiratory frequency (RF)during the 5 s period and 30 s before and after voiding onset were compared respectively. For cortical arousal definition the recommendations of the International Pediatric Work Group on Arousals(2005)were used. Results A total of 184 times of voiding were recorded. In preterm newborns,the VV and body movements rate were significantly lower compared with the term newborns[(21. 8 ± 7. 9)mL and(41 ± 21)% vs(26. 4 ± 8. 7)mL and(62 ± 19)% , t = 3. 75,4. 20,all P ﹤ 0. 05]. However,the VF and PRV were significantly higher in preterm newborns[(1. 7 ± 0. 9) mL and(3. 2 ± 1. 1)times vs(1. 2 ± 0. 7)mL and(2. 6 ± 0. 9)times,t = 2. 47,2. 38,all P ﹤ 0. 05]. Bladder voiding in these infants happened only during QS. In term newborns,HR frequency was higher during the 5 s interval before and after voiding onset when compared with the 30 s period before voiding onset[(152 ± 6)times/ min and(152 ± 5) times/ min vs(147 ± 6)times/ min,t = 5. 30,5. 76,all P ﹤ 0. 05]and the EEG frequency[(2. 6 ± 0. 1)Hz and (2. 6 ± 0. 1)Hz vs(1. 5 ± 0. 1)Hz,t = 70. 0,70. 0,all P ﹤ 0. 05]. While the HR and EEG frequency of preterm neo-nate was not changed before and after bladder voiding onset. The RF of both term and preterm neonates were not changed before and after bladder voiding onset. Conclusions The voiding patterns between term and preterm were sig-nificantly different and cortical arousal was found only in term neonates,which indicate the term newborns have better mature bladder function and development of nervous system.
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Objectives To investigate the rational treatment strategy of hepatoblastoma (HB) in children. Methods Clinical data and follow-up of 25 children with HB admitted from February 2009 to March 2013 were retrospectively analyzed. Results Twenty-five children with newly diagnosed HB (14 males and 11 females) were enrolled. The median age on diagnosis was 25 months (3-92 months);In 18 of 25 cases with complete resection of the primary tumor, 17 cases survived. Only 1 of 7 cases with incomplete resection survived. The survival rate in children with complete resection of primary tumor is significantly higher than those without complete resection (P<0.05). The survival rate in children of stage I or II is significantly higher than the children of stage III or IV (P<0.05). Conclusions Complete tumor resection is the cornerstone of therapy for long-term disease-free survival in HB patients. Treatment strategy remains to be further improved for children with recurrent and metastatic HB.