1.
Southeast Asian J Trop Med Public Health
;
1998 Dec; 29(4): 751-7
Artículo
en Inglés
| IMSEAR
| ID: sea-34685
RESUMEN
PCR optimization for differentiation of RSV subgroup A (RT-PCR-1) and RSV subgroup B (RT-PCR-2) were developed. Various conditions of RT-PCR-1 and RT-PCR-2 were summarized. These methods are highly specific and sensitive to differentiate RSV subgroup A and RSV subgroup B from the other respiratory viruses.
Asunto(s)
Preescolar , Métodos Epidemiológicos , Humanos , Lactante , Infecciones por Virus Sincitial Respiratorio/epidemiología , Virus Sincitiales Respiratorios/clasificación , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa/métodos , Sensibilidad y Especificidad , Tailandia/epidemiología
2.
Southeast Asian J Trop Med Public Health
;
1995 Dec; 26(4): 684-8
Artículo
en Inglés
| IMSEAR
| ID: sea-31506
RESUMEN
RT-PCR was compared with tissue culture to detect RSV from nasopharyngeal aspirates. RT-PCR was more sensitive and specific than tissue culture method. RT-PCR has sensitivity and specificity of 100% and 97%, respectively. The results indicate that RT-PCR can be used for detection of RSV in clinical specimens.