RESUMEN
The discovery of quorum sensing (QS) system and its critical role in bacterial virulence have revealed a new way to attack pathogenic bacterium. The pathogenecity of QS deletion mutants decreases significantly. Targeting bacterial QS system is a promising therapeutic approach to control infections and anti-microbial resistance. To obtain natural QS inhibitors from marine organisms, marine fungi (69 strains) were isolated from marine mollusca, and their extracts were screened using improved QSIS2 (Quorum Sensing Inhibitor Selector 2) assay and Chromobacterium violaceum CV026. To improve the efficiency of QSIS2 screening, 2,3,5-triphenyltetrazolium chloride (TTC) staining method was used. Extract from strain QY013 was found to have QS inhibitory activity. Further experiment indicated that pyocyanin in Pseudomonas aeruginosa PAOI and violacein in C. violaceum CV026 were reduced by QY013 extract, without affecting bacterial growth. Morphological and 18S rDNA sequence analysis revealed that strain QY013 was most closely related to Penicillium species. The above results suggest that active constituents from QY013 may be used as novel antimicrobial agents against bacterial infection.
Asunto(s)
Animales , Antiinfecciosos , Metabolismo , Farmacología , Fenómenos Fisiológicos Bacterianos , Hongos , Fisiología , Biología Marina , Moluscos , Microbiología , Penicillium , Metabolismo , Pseudomonas aeruginosa , Metabolismo , Virulencia , Percepción de Quorum , VirulenciaRESUMEN
OBJECTIVE: To investigate the shortage of small package of Chinese herbal pieces and to put forward some problems to be settled. METHODS: Some shortages of small package of Chinese herbal pieces were found out, and the improvement suggestion were put forward. RESULTS&CONCLUSION: The development of small package of Chinese herbal pieces requires the effective monitoring of drug regulatory agency.