Evaluation studies of antimicrobial activity on the leaf extracts of Achyranthes aspera Linn

Disc diffusion method was employed to determine the effect of methanol, acetone and chloroform extracts of the dried leaves of Achyranthes aspera against Staphylococcus aureus, Streptococcus fecalis, Klebsiella pneumoniae, Proteus vulgaris, Escherichia coli, Pseudomonas aeruginosa and Candida albicans. The chloroform extract shown to exhibit maximum potency against E. coli and Pseudomonas aeruginosa. Methanol extract showed moderate potency against E. coli and Candida species. Acetone extract was less effective against most of the species used except Streptococcus fecalis. Among all the extracts tested, none of the extract was found to be effective against gram positive S. aureus and gram negative Proteus vulgaris

Antimicrobial activity of extracts and isolates of the leaves of Plumeria alba Linn.

Disc diffusion method was employed to determine the effect of methanol, chloroform and acetone extracts of the dried leaves of Plumeria alba Linn. [Apocyitaceac] against fungi [Candida albicans] and bacteria [Bacillus subtilis, Proteus vulgaris Staphylococcus aureus. Pseudomonas aeruginosa, Streptococcus fecalis, Escherichio coil, Staphylococcus albus and Klebsiella pneumoniae]. The methanol and acetone extracts exhibited a prominent antimicrobial activity. The methanolic and acetone extracts were further fractionated by column chromatography to yield 2 pure isolates. The methanol pure component-2 [MPC[2]-50% Ethyl acetate:Methanol] and Acetone pure component-2 [APC[2]-70% Acetone:Ethyl acetate] have shown significant activity against all organisms used except Klebsiella

Anti-microbial activity of extracts and isolates of leaves of acalypha canescana linn

The in vitro antibacterial activity of the extracts and isolates of Acalypha canescana [leaves] have been studied against bacteria [Bacillus subtilis, Staphlylococcus aureus, Proteus vulgaris, Pseudomonas aeruginosa] and fungi [Candida albicans]. The total acetone extract, total methanol extract, total chloroform extract, total benzene extract and the fractionated two isolates of acetone extract and benzene extract have shown significant activity against the organisms used, almost comparable with the standard drugs Ciprofloxacin and Clotrimazolc. This in vitro testing also resulted in activity guided isolation of four antibacterial and antifungal principles from the leaves

Hepatoprotective effect of ethanol extract from leaves of Phyllanthus emblica in rats

In this study, phytochemical extraction was carried out on the leaves of Phyllanthus emblica using ethanol as the organic solvent. The antioxidant activity of ethanol extract from Phyllanthus emblica was evaluated by various biochemical marker parameters, including super oxide dismutase [SOD - anion radical scavenging], catalase [CAT - hydrogen peroxide scavenging], glutathione peroxidase [GPX], glutathione reductase [GRD - reducing power], glutathione transferase [GTS] and thio-barbituric acid reactive substances [TBARS] measurement. Hepato cellular damage was induced by intraperitoneal administration of CCl[4] in male albino Sprague-Dawley strain rats. The reduced levels of SOD, CAT, GPX and GRD with CCl[4] treated animals attain normal level, after administration of ethanolic extract of Phyllanthus emblica. However, the activity of GTS was significantly higher in CCl[4] treated animals, which were brought down towards normal level in herb treated rats. The increased concentration of TBARS with CCl[4] treated rats attains normal level after administration of ethanolic extract of the same plant. An antioxidant property appears to be predominantly responsible for this hepato-protective action of the drug