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Indian J Biochem Biophys ; 1996 Oct; 33(5): 363-70
Artículo en Inglés | IMSEAR | ID: sea-27813

RESUMEN

Soluble inorganic pyrophosphatase (EC 3.6.1.1) was isolated from chicken liver, RIR breed, to apparent homogeneity. The enzyme showed a molecular mass of 100 kDa as estimated by gel filtration and a subunit mass of 49 kDa on SDS-PAGE. The enzyme was very specific for pyrophosphate (PPi) and magnesium, and there was no measurable activity on replacing Mg2+ with Zn2+. At optimal conditions of assay, 50% of the enzyme activity was inhibited at 42 microM Ca2+, 70 microM fluoride and 0.91 mM Cd2+. There was a 50% inactivation of enzyme activity at 0.1 M guanidine hydrochloride (GuHCl). Kinetic analysis of GuHCl inactivation revealed 2 essential binding sites for this ligand. The enzyme showed allosteric behaviour with the substrate PPi and Mg2+. The apparent Hill coefficient of 1.47 and 1.48 for PPi and Mg2+, respectively indicate positive cooperatively. Hill plots also gave [S]0.5 of 0.177 mM and 2.5 microM for Mg2+ and PPi, respectively.


Asunto(s)
Animales , Cationes/farmacología , Pollos , Estabilidad de Enzimas , Pirofosfatasa Inorgánica , Cinética , Hígado/enzimología , Magnesio/farmacología , Masculino , Peso Molecular , Pirofosfatasas/química , Solubilidad , Especificidad por Sustrato
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