RESUMEN
In Bangladesh weather, environment and pattern of life style are significantly different from those of other developed as well as developing countries. So this makes us curious to determine the serum Dopamine-p-Hydroxylase [DBH] activity, its cofactors and other biochemical parameters in Bangladeshi diabetic patients at different ages of both sexes to determine their changes with age. The study was done in six different age groups of both sexes namely Group I: 10-15 years, Group II: 15-25 years, Group III: 25-35 years, Group IV: 35-45 years, Group V: 45-55 years and Group VI: 55+ years. DBH activity was increased in Groups I, II and decreased in Group VI of both sexes. DBH activity was variable in all other groups. One of the cofactors, ascorbic acid was slightly decreased in all groups. Another cofactor copper was variable. Among the parameters of lipid profiles, only the triacylglycerol level increased in all groups. Other biochemical parameters were variable. From our studies, we concluded that diabetes affects the DBH activity, its cofactors and other biochemical parameters
RESUMEN
The present study was designed to examine the influence of oocyte quality, culture media and gonadotropins on cleavage rate and development of in vitro fertilized buffalo Oocytes. Three experiments were conducted. In Experiment 1; oocytes were classified by number of cumulus cell layers and morphology of the oopbsm as Good, Fair or Poor. Oocytes were cultured for IVM, IVF and IVC in CRlaa medium. In Experiment 2, good quality oocytes were cultured for maturation in: [1] CR1aa; [2] CR2aa; [3] TCM-199; [4] MEM and [5] RPMI-1640, and then fertilized using frozen thawed buffalo spermatozoa in CRlaa. After fertilization, oocytes were cultured in the same medium used for maturation. In Experiment 3, oocytes were classified into 3 groups: Group [1] without gonadotropin and served as control; Group [2] in which IVM medium was supplemented with 10 micro g/ml FSH; Group [3] in which IVM medium was supplemented with 10 IU /ml PMSG. In all experiments, oocytes were kept at 38.5°C under 5% CO[2] for IVM, IVF, IVC and examined for cleavage and embryo development rates on day 3 and 8, respectively. Good and fair quality oocytes produced a higher [P <0.01] cleavage rate than poor quality oocytcs. Morula production rate was also higher [P < 0.01] for good as compared to fair quality oocytes. Embryo development with poor quality oocytcs was arrested at the 2 to 16-cell stage. In, Experiment 2, the cleavage rate was significantly higher [P < 0.05] in CR1aa than CR2aa. and significantly higher [P < 0.01] than TCM-199, MEM and RPMI-1640. The numbers of morulae and blastocysts were higher [P < 0.01] for oocytes cultured in CR1aa and CR2aa media than TCM-199 or MEM. In Experiment 3, the addition of FSH or PMSG to the maturation medium significantly increased [P < 0.01] cleavage and developmental rates of buffalo embryo compared to control media. In conclusion, the IVM of good quality buffalo oocytes in CR1aa or CR2aa medium and the addition of FSH or PMSG in maturation medium produced higher cleavage and developmental rates of IVF buffalo embryos