RESUMEN
Background and Aim: degenerative retinal diseases are among the common causes of blindness in the world. The purpose of this study was to investigate transplantation of neurosphere derived from bone marrow tissue into subretinal space in age related macular degeneration induced by injection of sodium iodate in animal model
Materials and Methods: 40 mg/kg of sodium iodate was injected into retro-orbital sinus of albino rats. Then histological investigation by flat-mount and hematoxylin and eosin staining was performed after 30 days. Bone marrow stromal stem cells isolated from albino rats femur, were cultured in the differentiation medium and induced into floating neurosphere. Differentiated cells were labeled with nuclear anti-BrdU and were transplanted into subretinal space. Seven days after injection, sections were prepared, and survival, migration and also arrangement of transplanted cells were investigated by immunohistochemistry
Results: three days after sodium iodate injection, the pathological changes such as increased autofluorescence, hypertrophy and multinuclearity in retinal pigmented epithelium were observed. Histological investigation showed disorganization of outer segment of photoreceptors and also changes in the retinal pigmented epithelium. Immunohistochemsitry findings, seven days after injection, showed that transplanted cells survived in subretinal space and could migrate into both retinal pigmented epithelium and the retinal layer and finally integrated with host tissue
Conclusion: due to accessibility, mesenchymal stem cells are regarded as a good source for transplantation. Potential of differentiation to neural linage and also survival ability and migration of these cells after transplantation could be regarded as a new way for the treatment of retinal degenerative diseases
RESUMEN
Background and Aim: the number and potential of proliferation and differentiation of bone marrow mesenchymal stromal cells decreases with age. These changes reduce efficacy of autologous transplantation in old people. The purpose of this study was to evaluate the effect of sodium selenite on telomere length and telomerase activity of bone marrow stromal cells [BMSCs] in aged rats
Material and Methods: the BMSCs collected from aged male rats were cultured and treated with different concentrations of sodium selenite for 72 h. We evaluated the effect of sodium selenite on the proliferation potential of these cells using trypan blue exclusion test. Then, we evaluated the efficacy of the effective concentration of sodium selenite on telomerase activity, telomere length and the related telomerase gene expression. Telomerase activity was assessed byPCR-ELSA method and telomere length, and its related gene expression was assessed by the real time PCR technique
Results: use of sodium selenite at the concentration of 100nM led to significant increase in the proliferation of BMSCs and decreased telomere length in the aged rats compared to the control group, although the difference was not significant. Telomerase activity and the related telomerase gene expression did not show any change
Conclusion: sodium selenite improved proliferation of BMSCs of the aged rats