Histochemical changes in pregnant rat's liver following repeated halothane exposure

Eighteen adult white rats were divided into 3 groups, 6 rats each [two males and four females]. The first group was exposed to 1.5% halothane for 20 minutes, every other day for 6 exposures. The rats were mated and exposed for another 10 exposures. The second group was given 0.1% phenobarbitone in milk starting one week before and during the period of exposure, which was similar to the first group. The third group was kept as a control. At the suspected date of delivery, the rats were sacrificed and specimens were taken from the mother's liver for estimation of the activities of the enzymes succinate dehydrogenase, acid phosphatase and alkaline phosphatase. Repeated halothane exposure decreased the activity of the enzymes succinate dehydrogenase, acid phosphatase and alkaline phosphatase. Treatment with phenobarbitone has the same effect on succinate dehydrogenase, but partially spared the activities of acid and alkaline phosphatases

Histochemical changes in pregnant rat's liver following repeated halothane exposure [1]

Eighteen adult white rats were divided into 3 groups, 6 rats each, 2 males. The first group was exposed to 1.5% halothane for 20 minutes, every other day, for 6 exposures. The rats mated and exposed for another 10 exposures. The second group was given 0. 1% phenobarbitone in milk, one week before and during the period of exposure, which similar to the first group. The third group was kept as a control. At the suspected date of delivery, the rats weresacrified and specimens were taken from the mother's liver for estimation of the activities of the enzymes succinate dehydrogenase, acid phosphatase and alkaline phosphatase. Repeated halothane exposure decreased the activity of the enzymes succinate dehydrogenase, acid phosphatase and alkaline phosphatase. Treatment with phenobarbitone has the same effect on succinate dehydrogenase, but partially spared the activities of acid and alkaline phosphatases

Histopathological changes in pregnant rat's liver following repeated halothane exposure [2]

Adulte white rats were divided into 3 groups, 6 rats each [2 and 4]. The first group was exposed to 1.5% halothane for 20 minutes every other day for 6 exposures. The rats were mated and exposed another 10 exposures. The second group was given 0.1% phenobarbitone in milk one week before, and during the period of exposure which was similar to the first group. The third group was kept as a control. At the suspected date of delivery, the rats were sacrified, and specimens were taken from the liver for haematoxylin and eosin stain, PAS reaction and Sudan black stain. Repeated exposure to halothane did not affect the pregnancy rate, but it resulted in hepatic focal and centrilobular necrosis with glycogen poverty and moderate lipid content. The portal spaces showed thick connective tissue and lymphocytic infiltration. Phenobarbitone reduced the pregnancy rate and resulted in 16.5% foetal mortality with more hepatic necrosis and increases in glycogen and lipid content

effect of suxamethonium administration on serum potassium level in bilharzial and non-bilharzial patients

This study was conducted on 4 groups of patients two of them were complaining of bilharzial hepatic fibrosis. Serum K level was estimated pre, 4, 8, 12 minute after administration of suxamethonium and also after halothane induction. There was significant increase in serum potassium level after four and eight minutes following suxamethonium administration as compared to that before suxamethonium. It returned to normal level after twelve minutes. In bilharzial patients there was no significant change in serum potassium level after four, eight and twelve minutes following suxamethonium injection. Serum potassium level in the second and third groups showed no significant change. There was significant decrease in serum potassium level after four minutes from halothane induction in bilharzial patients as compared to normal ones