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Clinics ; 67(2): 113-123, 2012. ilus, graf, tab
Artículo en Inglés | LILACS | ID: lil-614634

RESUMEN

OBJECTIVE: The establishment of the intestinal microbiota in newborns is a critical period with possible long-term consequences for human health. In this research, the development of the fecal microbiota of a group of exclusively breastfed neonates living in low socio-economic conditions in the city of São Paulo, Brazil, during the first month of life, was studied. METHODS: Fecal samples were collected from ten neonates on the second, seventh, and 30th days after birth. One of the neonates underwent antibiotic therapy. Molecular techniques were used for analysis; DNA was extracted from the samples, and 16S rRNA libraries were sequenced and phylogenetically analyzed after construction. A real-time polymerase chain reaction (PCR) was performed on the samples taken from the 30th day to amplify DNA from Bifidobacterium sp. RESULTS: The primary phylogenetic groups identified in the samples were Escherichia and Clostridium. Staphylococcus was identified at a low rate. Bifidobacterium sp. was detected in all of the samples collected on the 30th day. In the child who received antibiotics, a reduction in anaerobes and Escherichia, which was associated with an overgrowth of Klebsiella, was observed throughout the experimental period. CONCLUSION: The observed pattern of Escherichia predominance and reduced Staphylococcus colonization is in contrast with the patterns observed in neonates living in developed countries.


Asunto(s)
Femenino , Humanos , Recién Nacido , Masculino , Lactancia Materna , Bacterias/aislamiento & purificación , ADN Bacteriano/genética , Heces/microbiología , Intestinos/microbiología , /genética , Brasil , Bacterias/clasificación , Bacterias/genética , Bifidobacterium/genética , Bifidobacterium/aislamiento & purificación , Recuento de Colonia Microbiana , Clostridium/genética , Clostridium/aislamiento & purificación , Escherichia coli/genética , Escherichia coli/aislamiento & purificación , Pobreza , Análisis de Secuencia de ADN , Staphylococcus/genética , Staphylococcus/aislamiento & purificación
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