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Objective@#To explore the impact of smart physical education assignment on physical health of male university students, so as to provide theoretical support and practical references for physical health improvement of male university students and implementing smart sports assignments.@*Methods@#From September 2023 to January 2024, 317 sophomore male students from six Taekwondo elective classes at Hunan Institute of Engineering were selected and were randomly divided into an intervention group (n=157) and a control group (n=160). The intervention group was given sports assignments twice a week through smart means with an intervention duration of 15 weeks, each time for 25-35 minutes, in addition to the teaching according to the public course syllabus, while the control group was taught according to the public course syllabus. The physical and health indicators of both groups were tested before and after intervention,then the differences in various physical health indicators between two groups of students before and after intervention were compared through ttest and Mann-Whitney U test.@*Results@#After the intervention, the vital capacity, 50 m run, sitandreach, 1 000 m run, and pullup scores of the intervention group significantly improved compared to those before intervention. The scores improved from (3 918.27±737.34)mL, 7.88(7.53,8.45)s, 9.80(2.70,15.75) cm, 4.30(4.12,4.50) min and 3.00(0.00,7.50) times to (4 574.19±800.61) mL, 7.65(7.37,8.12)s, 17.20(11.80,21.55)cm, 4.13(3.58,4.31)min and 5.00(1.00,10.00) times,respectively (t/Z=-7.60, 2.61, -8.39, 5.62, -2.72, P<0.05). Before intervention, there was no statistically significant difference in physical health indicators between the intervention group and the control group (P>0.05).After intervention,the scores of the intervention group on the vital capacity,50 m run,sitandreach,1 000 m run and pullup, were significantly higher than those of the control group [(4 310.97±808.90)mL, 7.75(7.40,8.30)s, 14.10(8.42,17.87)cm, 4.29(4.08,4.45)min and 4.00(1.00,7.00) times] (t/Z=2.91, -4.55, -4.75, -4.15, 2.58, P<0.05).@*Conclusions@#Having 25-35 min smart physical education assignment twice a week can effectively improve physical health level of male college students. It is recommended to assign appropriate amount of smart sports homework to improve physical health level of college students, while ensuring the amount and intensity of physical activity in public physical education courses.
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Abstract Background Neonatal Intrahepatic Cholestasis (NICCD), as the early-age stage of Citrin deficiency involving liver dysfunction, lacks efficient diagnostic markers. Procalcitonin (PCT) has been identified as a biomarker for infection as well as various organ damage. This study aimed to explore the potential of PCT as a biomarker for NICCD. Methods In a single-center retrospective case-control study. Serum PCT concentrations before and after treatment of 120 NICCD patients, as the study group, were compared to the same number of cholestatic hepatitis patients, as the control group. The potential value of PCT to discriminate NICCD from control disease was further explored using Receiver Operating Characteristic (ROC) curve analysis and compared to those of other inflammatory markers. Results There was a significantly higher level of PCT in NICCD patients than in the control group. PCT concentrations were only weakly correlated with neutrophil counts and CRP levels (p ˂ 0.05). At a cut-off value of 0.495 ng/mL, PCT exhibited a significantly higher diagnostic value compared to other inflammatory markers for discriminating NICCD from the control, with a sensitivity of 90.8 % and specificity of 98.3 %. Conclusion PCT might be used as an initial biomarker to discriminate children with NICCD from another hepatitis disease.
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Objective:To explore the mechanism of gentiopicroside (GPS) in preventing acute liver injury induced by carbon tetrachloride (CCl<sub>4</sub>) in mice and its effect on the Toll-like receptor 4 (TLR4)/myeloid differentiation factor 88 (MyD88)/nuclear factor-<italic>κ</italic>B (NF-<italic>κ</italic>B) signaling pathway. Method:Sixty mice were randomly divided into a normal control group, a model group, a silymarin group (150 mg·kg<sup>-1</sup>), and high- (200 mg·kg<sup>-1</sup>), medium- (100 mg·kg<sup>-1</sup>), and low-dose (50 mg·kg<sup>-1</sup>) GPS groups, with 10 in each group. The mice in the groups with drug intervention were administered correspondingly by gavage at 10 mL·kg<sup>-1</sup>, and those in the normal control group and the model group receive an equal volume of distilled water, once per day. Ten days after administration, mice in the normal control group were subjected to the intraperitoneal injection of peanut oil (10 mL·kg<sup>-1</sup>) and those in other groups were injected with peanut oil (10 mL·kg<sup>-1</sup>) containing 0.12% CCl<sub>4 </sub>for the induction of acute liver injury model. After fasting for 16 hours, blood was collected from eyeballs and liver tissues were collected. Hematoxylin-eosin (HE) staining was used to observe the pathological changes of liver tissues. The content or activities of alanine aminotransferase (ALT), aspartate aminotransferase (AST), total bilirubin (TBIL), alkaline phosphatase (ALP), total superoxide dismutase (T-SOD), and <italic>γ</italic>-glutamyl transpeptidase (<italic>γ</italic>-GT) in the serum, malondialdehyde (MDA) and glutathione peroxidase (GSH-Px) in liver tissues were determined by biochemistry techniques. The levels of tumor necrosis factor-<italic>α</italic> (TNF-<italic>α</italic>), interleukin-1<italic>β</italic> (IL-1<italic>β</italic>), and interleukin-6 (IL-6) in liver tissues were measured by enzyme-linked immunosorbent assay (ELISA). Western blot was used to detect the protein expression of TLR4, MyD88, and NF-<italic>κ</italic>B in liver tissues. The expression of phosphorylated NF-<italic>κ</italic>B (p-NF-<italic>κ</italic>B) was detected by immunohistochemistry. Result:Compared with the normal control group, the model group showed increased levels of ALT, AST, ALP, TBIL, <italic>γ</italic>-GT, and MDA (<italic>P</italic><0.01), and blunted activities of T-SOD and GSH-Px (<italic>P</italic><0.01). Compared with the model group, the high- and medium-dose GPS groups exhibited declining levels of ALT, AST, ALP, TBIL, <italic>γ</italic>-GT, and MDA (<italic>P</italic><0.05, <italic>P</italic><0.01) and potentiated T-SOD and GSH-Px activities (<italic>P</italic><0.05, <italic>P</italic><0.01). Compared with the normal control group, the model group displayed elevated levels of TNF-<italic>α</italic>, IL-1<italic>β</italic>, and IL-6 in liver tissues (<italic>P</italic><0.01) and increased protein expression of TLR4, MyD88, and p-NF-<italic>κ</italic>B (<italic>P</italic><0.01). Compared with the model group, the high- and medium-dose GPS groups showed decreased TNF-<italic>α</italic>, IL-1<italic>β</italic>, and IL-6 content in liver tissues (<italic>P</italic><0.05, <italic>P</italic><0.01) and dwindled TLR4, MyD88, and p-NF-<italic>κ</italic>B protein expression (<italic>P</italic><0.05, <italic>P</italic><0.01). Conclusion:GPS possesses a protective effect on mice with acute liver injury induced by CCl<sub>4</sub>, and its mechanism of action may be related to the regulation of TLR4/MyD88/NF-<italic>κ</italic>B signaling pathway and inhibition of oxidative stress.