Dengue fever as a cause of acute febrile illness in Sharkiya

The prersent work aimed at detection of dengue as a cause of acute febrile illness in Sharkiya. One hundred patients complaining of fever, headache, myalgia and arthralgia were included in the present study. Acute and convalescent serum samples were collected from each patient and tested by indirect ELISA for the presence of dengue virus immunoglobulin G. Four-fold rise in antibody titre was found in sera eight patients"from acute to convalescent". The eight patients had leucopenia and none of them showed haemorrhagic manifestations. The present study showed that dengue fever can not be ignored as a possible cause of acute febrile illness in Sharkyia. So infection control measures must be taken to prevent its spread, especially in new countries, through people comming from endemic areas. Also, it is recommended to facilitate methods for laboratory diagnosis of dengue fever for early detection and treatment

role of hydroxamic acids in biochemical processes

Hydroxamic acids, a group of naturally occurring and synthetic weak organic acids of general formula RC[=O]N[R']OH, are widespread in the tissues of plants, in metabolites of bacteria and fungi, including complex compounds. Hydroxamic acids and their derivatives fulfill a variety of important roles in biology and medicine; here we provide a comprehensive brief review of the most basic medicinal chemistry and pharmacology of hydroxamate molecules

Cervical herpes simplex virus in Benha University Hospital clinic: cytologic, virologic and colposcopic study

Our objective was to determine the isolation rate of cervical HSV in relation to different clinical conditions and colposcopic findings, among the patients attending the outpatient clinic of Benha University Hospital and to find out the sensitivity and specificity of Papanicolaou [Pap] smear and direct fluorescence antibody stain [DFA] in relation to tissue culture. Our study comprised 250 patients. For each patient a Pap. smear was obtained and two endocervical scrapings were collected. The first one was used for staining with DFA. The second one was used for isolation of HSV-2 on monolayer of Buffalo Green Monkey kidney [BGM] cell culture. Colposcopy was done in all patients. Punch biopsy was taken from cases suggestive of squamous intraepithelial lesion. Out of the 250 patients, 36 [14.4%,] were positive for HSV by culture on BGM cells, 29 [11.6%] by DFA staining and 16 [6.4%] by Pap. smear. DFA has higher sensitivity [58.3% versus 33.3%,] and negative predictive value [93.2% versus 89.7%] than Pap smear. The virus isolation rate was not sign higher in the multigravida, multipara, patients with a history of 2 or more abortions, pregnant females, women using pills or intrauterine contraceptive device [IUCD], or patients with atypical transformation zone. The isolation rate of cervical HSV is 14.4% in the patients attending the outpatient clinic of Benha University Hospital. Pap. Smear is not useful in detection of HSV. The virus isolation rate was significantly higher in the age group [20-30 years], patients with a history of herpetic like lesions on the genitalia, patients with cervical ectopy and patients with infected typical transformation zone