RESUMEN
Objective: The effect of Lithium on central nervous system is well known but due to paucity of literature on scavenging effect of Lithium, this study was undertaken to see Lithium toxicity on cerebellar Purkinje neurons
Study Design: Experimental study
Place and Duration of study: This study was carried out at Animal House, JPMC, Karachi from April 2012 to June 2012
Materials and Methods: Fifteen male albino rats of 195-245 grams were selected and divided into three major groups A, B, and C. Each major group consisted of 5 animals
Time period of this study was 12 weeks. Group A served as control group which was given normal healthy lab diet and B was the Lithium Carbonate-treated group
Group C received Lithium carbonate in powder form and injection Methylcobalamin [B12] intraperitoneally
Lithium carbonate was given at a dose of 20mg/kg/day to group B and C for 12 weeks, and Methylcobalamin was injected at a dose of 200jng/kg/day/bwt. Purkinje cell count was performed with a counting reticule under light microscope
Results: The present study concluded that Lithium carbonate when administered the 12 weeks cause the significant decrease of Purkinje cell count and Methylcobalamin restored the cerebellar cell count
Conclusion: In the light of this study it was concluded that that Lithium carbonate causes significant permanent loss of permanent Purkinje cell neuron but Methylcobalamin provided neuroprotective effect and restored the cell count
RESUMEN
Objective: To observe the damaging effect of Lithium carbonate on Purkinje neuron in the light of available literature so this study was under taken to see the toxic effects of Lithium carbonate on permanent Purkinje neuron
Study Design: Experimental study
Place and Duration of study: This study was carried out at Animal House, BMSI, JPMC, Karachi from April 2012 to June 2012
Materials and Methods: Thirty male albino rats of 190-240 grams were selected and divided into two major groups [control] A and B [Lithium Carbonate-treated] comprising of 15 animals each. According to the time period of the study which was 2 weeks, 6 weeks and 12 weeks. Group A served as control group which was given normal diet and B was given Li2CO3 in powder form mixed in flour. Lithium carbonate was given at a dose of 20mg/kg/day for two, six and twelve weeks. At the end of 2[nd], 6[th] and 12[th] weeks the cerebellum was removed and fixed in 10% formal saline. After processing in paraffin five micron thick section were prepared four Purkinje cell count
Results: Group A revealed normal Purkinje cell count but the mean values of the Purkinje cell count in group B was highly significantly decreased p-value<0.001 as compared to group A
Conclusion: Our study concludes that acute and chronic ingestion of lithium carbonate at therapeutic level is detrimental to the survival of Purkinje cerebellar neuron and patients who are prescribed lithium carbonate should be monitored carefully
RESUMEN
Objective: To compare the thickness of cerebello cortical gray matter of albino rats after chronic ingestion of Lithium carbonate
Study Design: Prospective experimental study
Place and Duration of Study: This study was carried out in Animal House affiliated with atomy department BMSI, JPMC, Karachi from April 2012 to June 2012
Materials and Methods: Thirty male albino rats of 100-200 grams were selected and divided into two major groups A and B. Each major group consisted of 15 animals and my study was conducted according to the time period of the study which was 2 weeks, 6 weeks and 12 weeks. The control group purpose was served by Group A which was given lab diet and B was the Lithium treated group. Lithium carbonate [ADAMJEE PHARMACEUTICALS] was given at a dose of 20mg/kg/day for 2, 6 and 12 weeks. Cerebellar Gray matter thickness was measured at 2[nd], 6[th] and 12[th] weeks in the normal healthy control group and Lithium treated group
Results: Group B showed a progressive decrement of gray matter as the time period of study advance
Conclusion: The present study concluded that Lithium carbonate causes a significant decrease of cerebello cortical gray matter
RESUMEN
The effect of Lithium on the body and brain are well known but enough literature is not available on the effects of Lithium on body and Organ weights. So this study was undertaken to see the effect of Lithium on body and cerebellar weights. An experimental study. This study was conducted at the Anatomy Department BMSI, JPMC, Karachi. April 2012 to June 2012. Thirty male albino rats of 200-250 grams were selected and divided into two major groups A and B. Each major group consisted of 15 animals each groups was further divided into three sub-groups according to the time period of the study which was 2 weeks, 6 weeks and 12 weeks. Group A served as control group which was given lab diet and B was the Lithium treated group. Lithium carbonate was given at a dose of 20mg/kg/day for 2, 6 and 12 weeks. Body weights were recorded at the start and of each time period, but cerebellar, Organ weights were recorded at the end of 2[nd], 6[th] and 12[th] weeks. The body weights and Organ weights are recorded on digital electronic weighing scale. Group B showed a progressive decrease of body and cerebellar weights as the time period of study advanced. But relative cerebellar weights of group B increased with increasing time period of study. The present study concluded that Lithium carbonate causes a significant decrease of body and cerebellar weights