RESUMEN
<p><b>OBJECTIVE</b>To comparatively analyze the difference in the expression of 54 transcription factors in the hypothalamus using protein chips following the medication of Chinese drugs for Shen-tonification, Herba Epimedii, and Fructus Ligustri lucidi (FL) to aged rats.</p><p><b>METHODS</b>Wistar rats, aged 15 months of SPF grade, were randomized into three groups, three males and three females in each group. They were medicated with Herba Epimedii decoction (HED, 0.14 g/kg), Fructus Ligustri lucidi decoction (FLD, 0.12 g/kg), and distilled water, respectively, twice a day for 15 days. The rats were sacrificed at the morning of the 16th day 1 h after medication, and their hypothalamus was taken and made into homogenate under an ice-bath for detecting the expression of transcription factors with chip technique.</p><p><b>RESULTS</b>The expressions of signal transduction and transcription activation factor-6 (Stat-6) and androgen receptor (AR) were up-regulated, and those of pre-B transcription factor1 (Pbx-1), stat-1 and AP-2 were down-regulated in both HED and FLD treated groups, but these changes occurred mainly in female rats in the former while mainly in males in the latter.</p><p><b>CONCLUSIONS</b>Chinese drugs for Shen-tonification could impact the expression of transcription factors in the hypothalamus of aged rats, dominantly on the neuro-endocrine factors responsible for the growth and development. The effects of drugs for tonifying Shen-yang and for tonifying Shen-yin are different, which is probably one of the pharmacological mechanisms of the Shen-tonifying drugs.</p>
Asunto(s)
Animales , Femenino , Masculino , Ratas , Envejecimiento , Metabolismo , Regulación hacia Abajo , Medicamentos Herbarios Chinos , Farmacología , Hipotálamo , Metabolismo , Análisis por Matrices de Proteínas , Ratas Wistar , Factores de Transcripción , Metabolismo , Regulación hacia ArribaRESUMEN
The multiplex polymerase chain reaction (PCR) technique was applied to detect the SARS-CoV (severe acute respiratory syndrome-associated coronavirus) specific target cDNA fragments in the present study. The target cDNA fragments of SARS-CoV were synthesized artificially according to the genome sequence of SARS-CoV in GenBank submitted by The Chinese University of Hong Kong, and were used as simulated positive samples. Five primers recommended by World Health Organization (WHO) were used to amplify the fragments by single PCR and multiplex PCR. Three target cDNA fragments (121, 182 and 302 bp), as well as the three different combinations of any two of these fragments, were amplified by single PCR. The combination of these three fragments was amplified by multiplex PCR. The results indicated that the multiplex PCR technique could be applied to detect the SARS-CoV specific target cDNA fragments successfully.
Asunto(s)
Secuencia de Bases , Clonación Molecular , Cartilla de ADN , ADN Complementario , Genética , Datos de Secuencia Molecular , Reacción en Cadena de la Polimerasa , Métodos , Coronavirus Relacionado al Síndrome Respiratorio Agudo Severo , GenéticaRESUMEN
The fumarase from the culture of Aspergillus wentii F-871 was partially by means of precipitation with protamine sulphate, ammonium sulfate fractionation and column chromatography on Sephadex G-200, and then the concentrated enzyme solution was freeze-dried. The comparative enzyme activity of the fumarase was increased by 31.70 times and reached 24.6 U/mg, and the recovery was 36.64% . The optimal pH and temperature was 8.0 and 30℃, respectively. The pH stability of fumarase ranged 6.0 - 8.5, and more than 90% of the enzyme activity remained after incubated at 35℃ for 30 min.