RESUMEN
Objective: The present study was conducted to evaluate the effect of denervation on the filiform and the taste budbearing fungiform lingual papillae in adult rat
Methods: Sixteen adult albino rats were subjected to unilateral combined neurectomy of the chorda tympanilingual nerve. Differences between the intact and the denervated sides of the tongue were observed at 4 weeks [experimental group I ] and 12 weeks [experimental group ll] post-sectioning. Tongue specimens of both experimental groups were subjected to light microscopic study by Haematoxylirt and eosin [H and E] stain and immunohistochemical study using neuron specificenolase antibody. Scanning electron microscopic examination was also done
Results: Several histological and morphological alterations were demonstrated in papillae 0f the denervated side which were much influenced by the longer duration ofa'ertervation. Early postneurectomy [experimental group I], the fungiform papillae were the most affected. They appeared atrophied with sunken taste pores. Taste buds appeared disorganized with pyknotic nuclei and were weakly stained for neuron speci ICenolase. After 12 weeks postneurectomy [experimental group II], the fungiform papillae revealed a striking zliformlike appearance with absent taste buds on their summits. The filiform papillae exhibited disturbed orientations with heavily keratinized Surfaces
Conclusion: The structural and functional integrity of the lingual papillae are dependent on their intact trophic nerve supply. Extra care should be given to avoid the unnecessary nerve trauma during anesthesia or surgery
RESUMEN
Undescended testis is a common congenital abnormality in children that contributes to impaired fertility in adulthood. The pathogenesis of the reduced fertility seen in cryptorchidism has not been fully clarified. This work aimed at evaluating the role of germ cell apoptosis as well as serum inhibin B as prognostic markers of fertility potential. The study was carried on 32 children with unilateral undescended testis, divided into three age groups: group 1 [< 6 months], group 2 [6-12 months] and group 3 [12-24 months]. Control group included 22 age-matched children admitted for herniotomy. Histological assessment of apoptosis using semithin sections and immunohistochemical staining for Fas were done for testicular biopsy of cryptorchid patients. Hormonal assay of serum inhibin B, FSH, LH and testosterone of patients and controls were done. Blunted gonadotrophins surge was observed in cryptorchid infants [group 1], which was accompanied by defective transformation of gonocytes to adult spermatogonia. In groups 2 and 3, inhibin B was significantly lower in cryptorchid than their age- matched controls [P=0.014, P=0.01 respectively], while in these two groups testosterone level was very low in both patients and controls with no significant difference detected. Reduced inhibin B was parallel with high Fas expression and accelerated rate of germ cell apoptosis mostly in group two. In group 3, evidence of testicular atrophy was depicted. From the previous results, it could be concluded that the longer duration of maldescent carries more risk of impaired fertility potential, mainly through delayed germ cell maturation and enhanced apoptosis