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1.
The Korean Journal of Parasitology ; : 211-214, 2014.
Artículo en Inglés | WPRIM | ID: wpr-121884

RESUMEN

Epidemiological situation of taeniasis in Mongolia was assessed based on mitochondrial DNA identification of the parasite species. Multiplex PCR was used on a total of 194 proglottid specimens of Taenia species and copro-PCR and loop-mediated isothermal amplification (LAMP) assays were utilized for detection of copro-DNA of 37 fecal samples from taeniasis patients submitted to the Mongolian National Center for Communicable Diseases (NCCD) from 2002 to 2012. In addition, 4 out of 44 calcified cysts in beef kept in formalin since 2003 were evaluated for histopathological confirmation of cattle cysticercosis. All proglottid specimens and stool samples were confirmed to be Taenia saginata by multiplex PCR and by copro-PCR and LAMP, respectively. Cysts collected from cattle were morphologically confirmed to be metacestodes of Taenia species. T. saginata taeniasis was identified from almost all ages from a 2-year-old boy up to a 88-year-old woman and most prominently in 15-29 age group (37%, 74/198) followed by 30-44 age group (34.8%, 69/198 ) from 15 of Mongolia's 21 provinces, while cattle cysticerci were found from 12 provinces. The highest proportion of taeniasis patients was in Ulaanbaatar, the capital of Mongolia.


Asunto(s)
Adolescente , Adulto , Anciano , Anciano de 80 o más Años , Animales , Niño , Preescolar , Femenino , Humanos , Masculino , Persona de Mediana Edad , Adulto Joven , Bovinos/parasitología , Cisticercosis/epidemiología , ADN de Helmintos/genética , ADN Mitocondrial/genética , Heces/parasitología , Geografía , Carne/parasitología , Mitocondrias/genética , Mongolia/epidemiología , Enfermedades Desatendidas/epidemiología , Técnicas de Amplificación de Ácido Nucleico/veterinaria , Encuestas y Cuestionarios , Taenia saginata/genética , Taenia solium/genética , Teniasis/epidemiología
2.
The Korean Journal of Parasitology ; : 595-597, 2013.
Artículo en Inglés | WPRIM | ID: wpr-155349

RESUMEN

In December 2011, we reported an autochthonous case of Echinococcus multilocularis infection in a 42-year-old woman in Korea. The diagnosis was based on histopathological findings of the surgically resected liver cyst. In the present study, we evaluated the serological and molecular characteristics of this Korean E. multilocularis case. The patient's serum strongly reacted with affinity-purified native Em18 and recombinant Em18 antigens (specific for E. multilocularis) but negative for recombinant antigen B8/1 (reactive for Echinococcus granulosus). In immunoaffinity chromatography, the serum also strongly reacted with E. multilocularis and only weakly positive for E. granulosus. We determined the whole nucleotide sequence of cox1 (1,608 bp) using the paraffin-embedded cystic tissue which was compared with E. multilocularis isolates from China, Japan, Kazakhstan, Austria, France, and Slovakia. The Korean case showed 99.8-99.9% similarity with isolates from Asia (the highest similarity with an isolate from Sichuan, China), whereas the similarity with European isolates ranged from 99.5 to 99.6%.


Asunto(s)
Adulto , Animales , Femenino , Humanos , Anticuerpos Antihelmínticos/sangre , Antígenos Helmínticos/genética , Secuencia de Bases , Equinococosis Hepática/inmunología , Equinococosis Pulmonar/diagnóstico , Echinococcus granulosus/genética , Echinococcus multilocularis/genética , Complejo IV de Transporte de Electrones/genética , Mitocondrias/genética , Datos de Secuencia Molecular , República de Corea , Análisis de Secuencia de ADN
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