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Indian J Exp Biol ; 2014 Aug; 52(8): 825-834
Artículo en Inglés | IMSEAR | ID: sea-153766

RESUMEN

Dendrocalamus hamiltonii plants are slender and tall (15-25 m) thereby, rendering tagging, sampling and tracking the development of flowers difficult. Therefore, a reproducible system of in vitro flowering was established for tracking the stages of flower development. MS medium supplemented with 2.22 µM 6-benzylaminopurine, 1.23 µM indole-3-butyric acid and 2% sucrose was optimized as the flower induction medium (FIM) wherein 28 and 42 days were required for the development of gynoecium and androecium, respectively. Six distinct stages of in vitro flower development were identified, and the flowers were comparable with that of in planta sporadic flowers. Pollen viability of the in vitro flowers was higher than those of in planta ones. The in vitro system developed in the present study facilitates easy tracking of different stages of flower development under controlled environmental conditions. It can also be used for medium- or long-term storage of pollens and manipulation of in vitro fertilization.


Asunto(s)
Magnoliopsida/efectos de los fármacos , Magnoliopsida/crecimiento & desarrollo , Compuestos de Bencilo/farmacología , Flores/efectos de los fármacos , Flores/crecimiento & desarrollo , Técnicas In Vitro , Indoles/farmacología , Polen/efectos de los fármacos , Purinas/farmacología , Reproducción/efectos de los fármacos , Sacarosa/química
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