Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 2 de 2
Filtrar
Añadir filtros








Intervalo de año
1.
Novelty in Biomedicine. 2018; 16 (4): 162-166
en Inglés | IMEMR | ID: emr-206569

RESUMEN

Background: Genomic engineering of Escherichia coli is applied to design and produce recombinant proteins as the new drugs. The aim of this study was to CRISPR-Cas9 mediated capsule gene silencing in E. coli


Materials and Methods: We suppressed genes involved in capsule expression of E.coli by CRISPR cas9 process. The constructed E.coli was confirmed by microscopic smear, transmission electron microscopy and T7 phage influence assay


Results: The results were shown that the inhibition of capsule production was carried out successfully and there was not any capsule layer around the bacteria


Conclusion: E. coli without any capsule around may proper for replacement of it with other molecules in future

2.
Novelty in Biomedicine. 2017; 5 (3): 109-118
en Inglés | IMEMR | ID: emr-188713

RESUMEN

Background: Surface antigens [SAGs] of Toxoplasma gondii are known candidates for diagnostic tests and vaccines. The present study argues about the main necessary properties for determination and prediction of T-cell agretopes and B-cell epitopes of surface antigens of Toxoplasma gondii


Materials and Methods: Primary, secondary and tertiary structures of the proteins were analyzed by different methods. The three-dimensional structures were determined by use of ab initio method for prediction of discontinues epitopes. The agretopes and epitopes were predicted via several various web servers with different methods employed


Results: The results of in silico analyses showed that the regions 129-GAPAGRNNDGSSAPT-143 for protein p22, 234-SENPWQGNASSD-245 for protein p30 and 348-PGTEGESQAGT-358 for protein p43, have the highest immunogenic potential


Conclusion: We reached to three antigenic epitopes for cloning and protein expression. In following the purified polypeptide will be applied for diagnosis of Toxoplasma gondii

SELECCIÓN DE REFERENCIAS
DETALLE DE LA BÚSQUEDA