RESUMEN
Aim With SD rats as control, to observe the anxiety susceptibility of FH/Wjd rats. Methods The anxiety behavior of 3-month-old SD rats and age-matched FH/Wjd rats were evaluated by elevated plus-maze test and open field test. The contents of 5-hydroxytryptamine (5-HT), dopamine (DA) and its metabolite from the cortex were detected by high performance liquid chromatography with electrochemical detection, and the metabolic ratios of DA and 5-HT were calculated. The activities of catechol-O-methyltransferase (COMT) and monoamine oxidase (MAO), the contents of corticosterone (CORT) and adrenocorticotropic hormone (ACTH) were detected by enzymelinked immunosorbent assay in cortex. The expression of COMT was detected by polymerase chain reaction. The expression of all genes hippocampus was detected by mRNA-seq. Results As compared with SD rats, in FH/Wjd rats, the closed arms' distance and the total distance were significandy higher in elevated plus-maze; the central distance was significantly shorter, and the total distance was significantly longer in open field. The contents of DA,5-HT and DOPAC in cortex were significantly lower, and there was no significant difference in HVA and 5-HIAA. The ratio of HVA/DA and 5-HIAA/5-HT, the activity of COMT and MAO, the level of CORT and ACTH, the mRNA expression of COMTwere all higher. The differential genes of FH/Wjd rats and SD rats were mainly enriched in the neuroactive ligand-receptor interaction pathway. Conclusions Compared with SD rats, FH/Wjd rats have lower DA and 5-HT contents, hypermetabolism, hyperactivity of hypothalamic-pituitary-adrenal axis, abnormal expression of COMT and gene encoding neuropsychiatric system. Therefore, FH/Wjd rats have obvious anxiety characteristics.
RESUMEN
Anxiety disorders are a common mental illness that seriously endangered physical and mental health of human beings. The etiology of anxiety disorders is closely related to the abnormality of monoamines neurotransmitters, amino acids neurotransmitters and neuropeptides. The long-term use of anti-anxiety chemical drugs has some adverse effects, such as constipation, muscle relaxation, lethargy, tolerance and withdrawal symptoms. However, traditional Chinese medicines have advantages of multi-component, multi-target coordination, with less adverse reactions. Therefore, it is a promising prospect to develop novel anti-anxiety drugs from traditional Chinese medicines and formulas. This article reviewed some traditional Chinese medicines and formulas that can relieve anxiety symptoms. These include traditional Chinese medicines(Panax ginseng, Lycium ruthenium, Morus alba, Bupleurum plus dragon bone oyster soup, Chailong Jieyu Pills, and Naogongtai Formulas) with the effect on monoamine neurotransmitters, such as serotonin, dopamine, and norepinephrine; traditional Chinese medicines(Rehmannia glutinosa, Ziziphus jujuba Mill. var. spinosa, Jielv Anshen Decoction, Baixiangdan Capsules, Antianxietic Compound Prescription Capsules) with the effect on amino acid neurotransmitters, such as glutamic acid, γ-aminobutyrc acid; and traditional Chinese medicines(P. ginseng, Xiaoyao San, Shuyu Ningxin Decoction)with the effect on neuropeptide Y pathway, with the aim to provide theoretical basis for the further development of some novel and more effective anti-anxiety therapeutics from traditional Chinese medicine and formulas.