Study on application of hyperchem program for calculating the kinetics and optimal procedure of staining non--specific esterase of human lymphocyte

By using the hyperchem program, authors calculated quantitatively the change about the charge and stereo structure of molecules along various stages of the reaction of non-specific esterase (ANAE); Simultaneously, the changes of torsion, angle, energy, bond length were showed. That is evident to prove correlatively the kinetic mechanism of the electrophin substitute reaction. From the above calculations, the factors that affect to the optimum process for a highest effect of ANAE cytochemistry can be deduced

To establish and optimal process for of human monocyte by simplex method

Through the optimised research by simplex method, authors have found the optimal conditions of ANAE cytichemistry reaction such as the temperature of 37oC, pH=7.98, the time of 1 hour; especially with this method we can reduce the substance by 7-8 times compared other one

Test for stupefacient

The effective analysis of the stupefacient must be a sensitivity, specificity, and reliability, correct, prompt and easy to make. There are 2 kinds of stupefacient including natural and synthetic agents. The metabolism of the stupefacient and the collection of samples influenced on the results of analysis. The tests of the stupefacient are a test with the high sensitivity in the ligal aspect