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Braz. j. microbiol ; 43(3): 1042-1050, July-Sept. 2012. graf
Artículo en Inglés | LILACS | ID: lil-656673

RESUMEN

Total 95 isolates of Aureobasidium pullulans were isolated from different flowers and leaves samples, out of which 11 thermotolerant strains produced pullulan. One thermotolerant non-melanin pullulan producing strain, designated as RG-5, produced highest pullulan (37.1±1.0 g/l) at 42ºC, pH 5.5 in 48h of incubation with 3% sucrose and 0.5% ammonium sulphate in a non-stirred fed batch fermentor of 6 liters capacity. The two liters of initial volume of fermentation medium was further fed with the 2 liters in two successive batches at 5 h interval into the fermentor. The sterile air was supplied only for 10h at the rate of 0.5 vvm.


Asunto(s)
Estructuras de las Plantas/enzimología , Fermentación , Flores/enzimología , Hojas de la Planta/enzimología , Fungicidas Industriales/análisis , Hongos Mitospóricos/enzimología , Hongos Mitospóricos/aislamiento & purificación , Levaduras/aislamiento & purificación , Polisacáridos/análisis , Incubadoras , Métodos
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