1.
Experimental & Molecular Medicine
;
: 53-57, 1998.
Artículo
en Inglés
| WPRIM
| ID: wpr-192956
RESUMEN
Glyoxalase (GLO) II, which is a component of GLO system and catalyze the conversion of S-lactoyl-glutathione to D-lactate, was purified 1488 fold from rat liver by two steps of Affigel blue and carbobenzoxyglutathione-Sepharose 4B affinity chromatography. The molecular weight of the enzyme was estimated to be 29 kDa which is similar to those from other species. The sequence of N-terminal 9 amino acid residues was determined to be MGIRLLPAT. This was then used to synthesize degenerative primers. cDNA clone was isolated by first synthesizing cDNA from RNA and then PCR amplification. The sequence of cDNA clone was determined by serial sequencing analysis.
Asunto(s)
Ratas , Secuencia de Aminoácidos , Animales , Secuencia de Bases , Clonación Molecular , Estudio Comparativo , Hígado/enzimología , Datos de Secuencia Molecular , Ratas Sprague-Dawley , Análisis de Secuencia de ADN , Homología de Secuencia de Aminoácido , Tioléster Hidrolasas/aislamiento & purificación , Tioléster Hidrolasas/genética
2.
Korean Journal of Anatomy
;
: 104-115, 1992.
Artículo
en Coreano
| WPRIM
| ID: wpr-653553