Impact of morphine dependency on secondary intention wound healing in rat

Wound healing has always been among important and crucial subjects in medicine. Morphine dependency has also been a social and health problem in the Middle East. This study was aimed to investigate the effects of morphine dependency on pro-inflammatory and fibroblast cell recruitment, as well as reepithelialization and the revascularization processes involved in secondary intention wound healing in rats. A full-thickness wound [2×2 cm in diameters] was created on the dorsum of two groups of rats, a control group and a second group consisted of morphine dependent rats. During the first 14 days of post wounding the wound was excised consecutively at priorly planned days with peripheral margins of normal skin. The specimens were evaluated by two pathologists, who were blind to the study design, and the cellular population, re-epithelialization and revascularization were reported by them. Histological examination of the wound tissue showed evidence of increased population of fibroblasts and a plateau or decreased recruitment of macrophage and neutrophile cells. In the dependent group re-epithelialization was observed to be enhanced significantly in comparison to the control group while having an inhibitory effect on revascularization. The present study demonstrates that morphine dependency enhances re-epithelialization as well as tissue recruitment of fibroblasts; thereby probably enhancing secondary intention wound healing

Study of laser assisted hatching for fertility outcome in intra cytoplasmic sperm injection and IVF cycles

Implantation of transferred embryos in the uterus after IVF_ICSI cycles is an important process that less information about it is available. Zona hatching method is a suggested approach for this goal. The aim of this research is about performance of zona hatching by laser. In this study 32 patients that enrolled had IVF/ICSI cycles depend on inclusion criteria of study that they included patients who were more than 35 years old, at least one previous failure of IVF, thickness or hardness of zona pelucida, the laser hatching performed on them. Then the pregnancy test [beta-subunit] was done and patients with a positive result are followed with vaginal ultra sonography. Our successful results belonged to the patients who had more than 35 years old. So 50% of pregnancy was positive and then 20% about the zona pelucida thickness and minimum results were about the previous failure of IVF/ICSI cycles. Laser hatching on the embryos of women more than 35 years old with IVF/ICSI cycle will optimize pregnancy outcomes