Histopathological evaluation in experimental envenomation of dogs with Crotalus durissus terrificus venom

The present work evaluated histopathological aspects in experimental envenomation of dogs with Crotalus durissus terrificus venom. Twenty-eight mixed breed adult dogs were divided into three groups of seven animals each: Group I - only venom; Group II - venom + 50ml antiophidic serum + fluid therapy; Group III - venom + 50ml antiophidic serum + fluid therapy + urine alkalization. Lyophilized venom of Crotalus durissus terrificus was reconstituted in saline solution and inoculated subcutaneously at the dose of 1mg/kg body weight. Three animals of each group were subjected to euthanasia, and their muscular tissue, brain, spleen, kidneys, heart, lungs, stomach, small and large intestines, and popliteal lymph node fragments were collected for histopathological evaluation. There was myonecrosis in the inoculated limb, renal tubular degeneration, lymphoid hyperplasia of spleen, and unspecific reactive hepatitis. These results show the antigenicity and action of the venom on the immune system.

Experimental envenomation with Crotalus durissus terrificus venom in dogs treated with antiophidic serum - part II: laboratory aspects, electrocardiogram and histopathology

The present work shows laboratory aspects, electrocardiogram and histopathology results during experimental envenomation by Crotalus durissus terrificus in dogs treated with antiophidic serum. Twenty-one dogs were divided into three groups of seven animals each. Group I received 1mg/kg venom (sc); Group II received 1mg/kg venom (sc), 50mg antiophidic serum (iv) and fluid therapy including 0.9 percent NaCl solution (iv); and Group III received 1mg/kg venom (sc), 50mg antiophidic serum (iv) and fluid therapy including 0.9 percent NaCl solution containing sodium bicarbonate diluted to the dose of 4mEq/kg. Urinalysis showed brown urine, proteinuria, occult blood and myoglobinuria. Respiratory acidosis and hypotension were also observed. At the venom inoculation site, there was discreet edema, popliteal lymph node response, musculature presenting whitish areas and necrotic myositis with myoregenerative activity. There was not evidence of electrocardiographical and biochemical alterations.

Biopsia hepática em cäes: relaçäo entre qualidade da amostra e grau de conclusäo do diagnóstico / Hepatic biopsy in dogs: relationship between sampling and degree of diagnosis conclusiveness

O presente estudo objetivou analisar a qualidade do diagnóstico de biopsias colhidas com agulhas tipo aspirativa e cortante, comparando-as ao exame histológico post-mortem do fígado de cäes portadores de afecçöes hepáticas. Foram utilizados 60 cäes, sem distinçäo de sexo, raça ou idade. As biopsias foram procedidas com agulhas tipo Menghiniômicron (de natureza aspirativa) e agulhas tipo Tru-Cutômicron (de natureza cortante). As biopsias foram colhidas utilizando-se a técnica percutânea transabdominal. O diagnóstico referente à agulha tipo Tru-Cutômicron näo diferiu do diagnóstico referente à agulha tipo Menghiniômicron. O referente à agulha tipo Tru-Cutômicron foi o que apresentou maior índice de concordância, 88,3 por cento de acertos. A agulha tipo Tru-Cutômicron apresentou excelentes resultados independente da natureza das lesöes, enquanto que a agulha tipo Menghiniômicron falhou nos casos de fibrose

Inflammatory pseudotumor of the urinary bladder in dogs: two cases

Relata-se a ocorrência de dois casos de pseudotumor inflamatório na bexiga de cäes, enfatizando a importância de reconhecer o caráter benigno dessas lesöes, evitando uma abordagem terapêutica agressiva como conseqüência de um diagnóstico equivocado