Amelioration potential of Withania sonmifera root extract on hexavalent chromium induced micronucleus in Channa punctatus (Bloch, 1793)

Aim: This study aimed to infer the ameliorative potential of Withania somnifera (‘Ashwagandha’) against hexavalent chromium induced micronuclei in Channa punctatus.Methodology: After laboratory acclimatization of 15 days, C. punctatus (12.20 cm, 42 g) were maintained in six groups. Group I, served as control. Fishes of groups II and III were separately exposed to root extract of W. somnifera (3 mg l-1) and 96 hr-LC50/10 of Cr (VI), 7.89 mg l-1, respectively, for 24, 48, 72 and 96 hr. Contrarily, the fish of groups IV, V and VI were exposed to 7.89 mg l-1 of Cr (VI) along with increasing concentrations of root extract of W. somnifera (1, 2, 3 mg l-1), respectively. Induction of micronuclei was assessed in fishes of all the six groups after stipulated exposure periods. Results: A significant induction (p<0.05) in micronuclei frequency was observed in Group-III as compared to the control. On contrary, there was a significant (p<0.05) decrease in frequency of micronuclei induction with increasing concentrations of root extract of W. somnifera, as compared to Group-III, after stipulated exposure periods in a dose and time-dependent manner. Interpretation: Preliminary investigations evinced that the root extract of W. somnifera has enough ameliorative potential against short term sub-lethal exposure to Cr (VI) induced genomic instability, i.e., micronuclei induction in C. punctatus.

Investigations on remedial role of Rauwolfia serpentina root extract against carbofuran formulation induced genotoxicity in Channa punctatus

Aim: To investigate the remedial potential of Rauwolfia serpentina root extract against genotoxic alterations induced by exposure of carbofuran formulation in freshwater teleost, Channa punctatus. Methodology: Ten days acclimatized fish were categorized in three groups, Group 1 (control), Group 2 (0.09 mg l-1 carbofuran formulation) and group 3 (0.09 mg l-1 carbofuran formulation +10 ppm ethanolic extract of Rauwolfia serpentina). Genotoxic alterations were recorded in terms of single cell gel electrophoresis (SCGE) and micronucleus (MN) assay in blood cells. The variation in comet tail length and micronuclei frequencies were compared among Group 1, 2 and 3 after 24, 48, 72 and 96 hr. Results: A significant (p<0.05) increase was observed in comet tail length and micronuclei induction in carbofuran formulation exposed group. The longest comet length and peak of micronuclei frequencies were observed after 96 hr of carbofuran formulation exposure. However, an appreciable and gradual decline in both frequencies of micronuclei and comet tail length were observed in group 3 (combined Carbofuran formulation and Rauwolfia serpentina root extract) in comparison to group 2 (Carbofuran formulation). Interpretation: The study, thus, demonstrates ameliorative potential of Rauwolfia serpentina root extract against carbofuran formulation induced genotoxicity in fish.

Impact of copper on haematological profile of freshwater fish, Channa punctatus.

The present study was to evaluate heavy metal toxicity stress symptoms in fish blood during long-term exposure of sublethal concentration of copper sulphate (CuSO4). The effects of copper on various heamatological parameters were evaluated exposing Indian fresh water fish, Channa punctatus to a sub-lethal concentration of copper sulphate (0.36 mg l(-1)) for different periods. Exposure of fish to copper showed a significant decrease in the haemoglobin (Hb) content from 10.73 to 6.60%, red blood cells (RBC) from 2.86 to 1.84 x 10(6)/mm3 and packed cell volume (PCV) from 31.00 to 23.33% at the end of 45th day as compared to control. Whereas the white blood cells (WBC) increased from 60.00 to 92.48 x10(3)/mm3, clotting time (CT) from 27.66 to 43.00 second, erythrocyte sedimentation rate from 5.0 to 13.66 mm/hr and mean corpuscular volume from 108.11 to 126.85 cmu, significantly, with increase in exposure periods. Although mean corpuscular haemoglobin concentration (MCHC) and mean corpuscular haemoglobin (MCH) values showed significant increase during 15 and 30 days exposures, both the values were found significantly increased at the end of the experimental period. The differential leucocytes count (DLC) showed a significant increase in the populations of lymphocytes and eosinophils, whereas neutrophils, monocytes and basophiles were found decreased after exposure to copper.

Histopathological changes in testis of the freshwater fish, Heteropneustes fossilis (Bloch) exposed to linear alkyl benzene sulphonate (LAS).

The objective of this study was to use the freshwater food fish, Heteropneustes fossilis, as a model to evaluate the concentration-dependent effects of LAS on the testicular structure through short-term static bioassays. Concentration mediated histopathological leisions were observed in testis of H. fossilis, treated with four different concentrations of LAS for 24, 48, 72 and 96 hr Inference drawn from the study is that the cytotoxic damage is more pronounced in fish exposed to higher concentrations of LAS for shorter durations than lower concentrations of LAS for longer durations. Gross damage of germinal epithelium, inflammatory response, intertubular vacuolations and contraction and condensation in the cells of tubules under all sets of intoxication and exposures are quite suggestive of reproductive impairment leading to delayed gonadal maturity.

Evaluation of genotoxic potential of chromium (VI) in Channa punctata fish in terms of chromosomal aberrations.

Chromium, a widely recognized carcinogenic, mutagenic and redox active metal, is released into aquatic environments by electroplating, tannery and textile industries. Elevated concentrations in sediments and interstitial waters are well documented. Fishes dwelling in chromium waste infested waters are presumed to be affected by its deposits. To evaluate the genotoxic potential of chromium [Cr(VI)] on aquatic bio-system, bottom feeding fishes, Channa punctata, as model fish, were exposed to [Cr(VI)]. The chromosomal aberration test (CAT) was used as biomarker of [Cr(VI)] induced toxicity. The fish were divided into three groups:Group I non-treated controls; group II positive controls, treated with an intra-muscular injection of mitomycin-C at 1 mg/kg body wt; group III exposed to a sublethal concentration (7.689 mg/l) of [Cr(VI)], dissolved in the water. For CAT estimation, short term static bioassays were conducted and samples were collected from the kidneys of fish after 24, 48, 72, 96 and 168 hrs of exposure. The remarkable chromosomal aberrations recorded in the present investigation included chromatid breaks, chromosome breaks, chromatid deletions, fragments, acentric fragments, and ring and di-centric chromosomes, along with chromatid and chromosome gaps. A significant increase in chromosomal aberrations was observed after 72 hrs of [Cr(VI)] exposure. The present study, thus reveals that even for acute exposure, [Cr(VI)] is a genotoxic agent for C. punctata.

Effect of linear alkyl benzene sulphonate on erythrocyte sedimentation rate of teleost fish, Heteropneustes fossilis.

ESR increased at all concentrations and exposures to LAS. However, increases were more pronounced at LC50 for 24 h and minimum at the end of 96 h.

Haematotoxic effect of linear alkyl benzene sulphonate (LAS) in fish, Heteropneustes fossilis.

It was observed that clotting time decreased with the increased concentrations of LAS. However, the decrease was more pronounced at LC50 for 24 h and at the end of 96 h LC50, it was very close to control values.

Impact of linear alkyl benzene sulphonate (LAS) on phosphatase activity in testis of the teleostean fish, Heteropneustes fossilis (Bloch).

Fishes are sensitive indicators of pollutants present in water.These pollutants cause various physical and physiological alterations in fishes. In the present work alteration in the activity of acid and alkaline phosphatase was evaluated in testicular tissue of fresh water fish Heteropneustes fossilis exposed to LC50 value of linear alkyl benzene sulphonate (LAS) for different exposure periods [24 h, 48 h, 72 h and 96 h] With increase in the concentration of chemical LAS, the activity of acid phosphatase (ACP) was reported elevated while a significant fall in the activity of alkaline phosphatase (ACP) was recorded for same exposure period. Elevated activity of ACP, one of the important hydrolases of lysosomes, is quite suggestive of bringing about gross necrosis and dysarchitecture. ALP is involved in various metabolic activities including gonadal maturation and as such decreased activity of this enzyme is definitely one of the important causative factors for reproductive impairment of the fish.

The role of glucocorticoid pulse therapy in pulmonary involvement in leptospirosis.

OBJECTIVES: This study discusses incidence and clinical profile of pulmonary involvement in leptospirosis in South Gujarat. It also tries to evaluate the effect of high dose glucocorticoid pulse therapy (GPT) on it. METHOD: A study was carried out on hundred and two patients of suspected leptospirosis, referred to Government Medical College, New Civil Hospital, Surat between June 99 to September 99. The incidence, clinical profile, and specific investigations were studied in patients having pulmonary involvement. Some of the patients were given high dose glucocorticoid pulse therapy. Their outcomes were compared with those who had not been given glucocorticoid pulse therapy. RESULTS: Out of seventy seven seropositive patients 13 (16.8%) developed pulmonary involvement. Mortality was two out of eight patients in the group that received GPT and four out of five patients in the group that did not receive GPT. Two patients who died in the steroid treated group received the drug after 12 hours of onset of dyspnea. CONCLUSIONS: High dose GPT should be given as early as possible after the onset of dyspnea to all the patients with pulmonary involvement in leptospirosis. Further studies are required to establish the GPT as a standard regimen in treatment of pulmonary involvement in leptospirosis.