Asunto(s)
Humanos , Recién Nacido , Lactante , Sustitutos de la Leche Humana , Ambiente de Instituciones de Salud , Hospitales , Enterobacter , CronobacterRESUMEN
Abstract Poultry meat is a food product that usually carries high rates of microbial contamination, including foodborne pathogens. The poultry industry has established different systems to minimize these hazards. In recent years, extensive literature has demonstrated the antimicrobial activity of different contact surfaces made of copper to effectively reduce microbial loads. The aim of the present study was to evaluate the antibacterial effect of copper surfaces on the transmission of two foodborne pathogens - Salmonella enterica and Listeria monocytogenes - and a poultry native microbiota bacterial species - Enterobacter cloacae. We also evaluated the impact of the poultry meat matrix on the antimicrobial activity of a copper surface. Our results indicated that copper surfaces reduced the bacterial load quickly (<than 4 min) when the microorganisms were exposed to polished copper surfaces. Even when bacteria were inoculated on copper surfaces soiled with the organic matrix (washing water from poultry carcasses) and survival rates were significantly higher, an antimicrobial effect was still observed. Survival rates of two microorganisms simultaneously exposed to copper did not show significant differences. We found an antimicrobial effect over pathogenic and non-pathogenic microorganisms. Results suggest a potential role for copper surfaces in the control of microbiological hazards in the poultry industry.
Asunto(s)
Animales , Aves de Corral/microbiología , Cobre/farmacología , Carne/microbiología , Antibacterianos/farmacología , Cadáver , Contaminación de Alimentos/análisis , Salmonella enterica/efectos de los fármacos , Microbiota/efectos de los fármacos , Listeria monocytogenes/efectos de los fármacosRESUMEN
Abstract Poultry meat is a food product that usually carries high rates of microbial contamination, including foodborne pathogens. The poultry industry has established different systems to minimize these hazards. In recent years, extensive literature has demonstrated the antimicrobial activity of different contact surfaces made of copper to effectively reduce microbial loads. The aim of the present study was to evaluate the antibacterial effect of copper surfaces on the transmission of two foodborne pathogens Salmonella enterica and Listeria monocytogenes and a poultry native microbiota bacterial species Enterobacter cloacae. We also evaluated the impact of the poultry meat matrix on the antimicrobial activity of a copper surface. Our results indicated that copper surfaces reduced the bacterial load quickly ( than 4 min) when the microorganisms were exposed to polished copper surfaces. Even when bacteria were inoculated on copper surfaces soiled with the organic matrix (washing water from poultry carcasses) and survival rates were significantly higher, an antimicrobial effect was still observed. Survival rates of two microorganisms simultaneously exposed to copper did not show significant differences. We found an antimicrobial effect over pathogenic and non-pathogenic microorganisms. Results suggest a potential role for copper surfaces in the control of microbiological hazards in the poultry industry.
Asunto(s)
Lactante , Humanos , Diarrea Infantil/microbiología , Escherichia coli/patogenicidad , Infecciones por Escherichia coli/microbiología , Plásmidos/efectos de los fármacos , Farmacorresistencia Microbiana , Enfermedad Aguda , Escherichia coli/aislamiento & purificación , Antibacterianos/farmacologíaRESUMEN
Se estudió la sensibilidad de los antibióticos de 38 cepas enteropatógenas de Escherichia coli 0111 provenientes de niños menores de dos años, 29 obtenidas de pacientes con diarrea, las restantes de sujetos asintomáticos. Se encontró que 55% de las cepas eran resistentes a 3 o más antimicrobianos simultáneamente; de ellas todas lo eran a ampicilina, 90% a kananicina y carbenicilina y alrededor de 80% a estreptomicina y cefalotina; todas las cepas eran sensibles a furazolidona y 90% a gentamicina. Entre las cepas multirresistentes, 34% eran capaces de transferir, mediante conjugación, 2 a 6 determinantes de resistencia. El peso molecular de los plasmidios asociados a la transferencia flutuaba entre 41 y 80 Megadaltons (Md), predominando el de 54-55 Md. No se encontraron diferencias significativas en la frecuencia de multirresistencia y la capacidad de transferir entre cepas provenientes de niños con diarrea y asintomáticos