Resultados perinatales en pacientes complicadas con rotura uterina intraparto / Perinatal outcomes in patients complicated with uterine rupture in labor

RESUMEN Introducción: La perforación del músculo uterino que se presenta en embarazos cercanos al término es una condición grave que condiciona sufrimiento fetal agudo y/o muerte del producto de la concepción; además, pone en peligro la salud de la paciente. Objetivos: determinar la prevalencia de rotura uterina en pacientes con trabajo de parto y hallar las causas probables en relación a la edad y paridad. Metodología: diseño observacional, retrospectivo, transversal, realizado en gestantes en trabajo de parto que concurrieron al Hospital Materno Infantil San Pablo, durante el periodo de enero de 2005 a diciembre de 2007. Resultados: Entre 1094 fichas evaluadas, se halló una prevalencia de rotura uterina intraparto de 4,11%. En relación al cuadro clínico, 53,3% fueron casos asintomáticos. El tratamiento más frecuente fue la histerorrafia 88%. La edad gestacional más frecuente en que se produjo el evento fue entre 37 a 40 semanas 55%. La mayoría de los fetos tuvieron tuvo un peso normal entre 2500 y 3999 gr. 95%. Conclusiones: la rotura uterina tuvo una prevalencia de 4%. La mayoría de las gestaciones al momento de la RU eran de término y en la minoría de los casos eran de edad gestacional muy temprana. El peso de los productos se encontraba mayormente en rango considerado normal. Predominaron los casos de rotura parcial e incompleta.

ABSTRACT Introduction: perforation of the uterine muscle that occurs in near-term pregnancy is a serious condition that determines acute fetal distress and / or death of the fetus, in addition, endangers the health of the patient.Objectives: To determine the prevalence of uterine rupture in patients in labor and find probable cause in relation to age and parity. Methodology: Observational, retrospective, transversal study, performed in pregnant women in labor who attended the Hospital Materno Infantil San Pablo, during the period January 2005 to December 2007. Results: Between 1094 patients tested, we found a prevalence of intrapartum uterine rupture of 4.11%. In relation to the clinical manifestations, 53.3% were asymptomatic. The most common treatment was hysterorrhaphy (88%). The most common gestational age in which the event occurred was between 37 to 40 weeks (55%). Most fetuses had had normal weight between 2500 and 3999 gr (95%). Conclusions: Uterine rupture had a prevalence of 4%. The majority of pregnancies was at term and in the minority of the cases were very early gestational age. The weight of the products was mostly range considered normal. It predominant cases of partial and incomplete rupture.

Polymorphism of locus DRB3.2 in populations of Creole Cattle from Northern Mexico

The polymorphism of locus BoLA-DRB3.2 of the Major Histocompatibility Complex was evaluated in two northern Mexican Creole cattle populations, Chihuahua (n = 47) and Tamaulipas (n = 51). The BoLA-DRB3.2 locus was typed by amplification and digestion with restriction endonuclease enzymes (PCR-RFLP). Fifty-two alleles were detected (28 previously reported and 24 new ones). In the Chihuahua population, 18 alleles and 5.5 effective alleles were found, while in the Tamaulipas population there were 34 and 10.8, respectively. The allele frequencies ranged from 0.011 to 0.383 in Chihuahua and from 0.010 to 0.206 in Tamaulipas. The frequencies of the new alleles in both cattle populations were low (0.010 to 0.053). The expected heterozygosity was 0.827 and 0.916, respectively, for the Chihuahua and Tamaulipas populations. Both populations presented a heterozygote deficit: [Chihuahua FIS = 0.1 (p = 0.019) and Tamaulipas FIS = 0.317 (p < 0.001)]. In conclusion, this study showed that the Mexican Creole cattle have many low-frequency alleles, several of which are exclusive to these populations. Genetic distances obtained show that the Mexican Creole cattle population is composed of independent populations, far apart from other South American Creole populations.

Genetic polymorphism of the alpha s1-casein locus in five populations of goats from Mexico

With the objective of estimating allele frequencies, and testing for population divergence for the CSN1S1 locus, genotypes of animals from five goat populations; Saanen (n = 97), Alpine (n = 81) Toggenburg (n = 92), local goats with external appearance similar to the Murciana-Granadina breed from Central Mexico (n = 26) and heterogeneous local animals denominated Mosaico Lagunero (n = 30), from Northern Mexico, were identified using PCR and Xmn1 PCR-RFLP methodology. For Saanen, Alpine and Toggenburg, the sum of E and F alleles had the largest frequencies (from 0.468 to 0.789), while for the groups local Murciana-Granadina and Mosaico Lagunero the sum of the most frequent allelic groups (A and B), were 0.385 and 0.533 respectively. Both local Murciana-Granadina and Mosaico Lagunero populations showed heterozygote excess (P < 0.08). The percentage of the total genetic variation (F ST) explained by population differences was 5.16. There was genetic differentiation for most pair comparisons between populations (P < 0.05), excepting for Alpine versus Toggenburg, and Toggenburg versus Mosaico Lagunero (P > 0.05). For Saanen and Alpine the frequencies of alleles E and F were similar to the same breeds previously analyzed in Europe. Therefore there are opportunities of increasing the frequency of the strong alleles for protein content Gene Assisted Selection (GAS) in these two breeds. For Toggenburg the most frequent allelic groups were F (0.32) and B (0.21). Results indicate differentiation between most populations for this locus. Moreover, heterozygote excess in local populations indicated breed admixture.