RESUMEN
Background: Resistant strains of Pseudomonas aeruginosa to imipenem was medical treatment problem, especially in burnt units of hospitals
Objectives: This study was conducted to evaluate the antimicrobial effect of Fe[2]O[3] nanoparticles alone and functionalized with imipenem on P. aeruginosa starins producing metallo beta-lactamases [MBL]
Materials and Methods: A disk diffusion method was used to isolate a clinical P. aeruginosa producing Metallo beta-lactamases with imipenem resistance. The minimum inhibitory concentration [MIC] of Fe[2]O[3] nanoparticles and imipenem were calculated against the bacteria. The antimicrobial effect of nanoparticles functionalized with the antibiotic was determined. Standard strain of P. aeruginosa ATCC: 27853 was used as control
Results: The clinical sample was resistant to imipenem [up to 28 micro g.mL[-1]]. Similarly, MIC of the nanoparticles against the isolate was 160 micro g.mL[-1]. Subsequently, the combination of 16 pg.mL[-1] of antibiotic with 80 micro g.mL[-1] of Fe[2]O[3] nanoparticles were able to inhibit the growth of the isolate
Conclusions: Fe[2]O[3] nanoparticles functionalized with imipenem can impair antibiotic resistance mechanisms of bacteria as it can make the imipenem resistant the aforementioned bacterium more susceptible to weaker concentrations of antibiotic. It also has its own antibacterial effect in certain concentrations
RESUMEN
To compare culture methods with multiplex PCR technique for identification of Brucella abortus and Brucella melitensis from suspicious patients with clinical history of brucellosis and positive serological test [Rose Bengal test and serum agglutination test]. In this study, 160 blood samples from patients suspected of Brucellosis with high serum titers of 1/80 were studied. All samples were cultured in Brucella-specific media. Brucella species were identified by using microbiological methods. DNA was extracted with Phenol-chloroform DNA extraction method. IS711 was amplified simultaneously using three specific primers and obtained patterns were analyzed. From 160 samples, 47.5% [76] were culture positive cases from which 43 cases were B. melitensis and 33 were B. abortus With the PCR technique 108 were detected positive from which 45.3% were B. abortus and 54.6% were B. melitensis. It should be noted that all 76 samples with positive culture were also identified by PCR. Generally, use of the molecular technique multiplex PCR in addition to increased speed and accuracy and less false results than bacterial culture method, is able to identify different species of brucella. This will facilitate the treatment process