Natural Sources of Gooseberry Component used for Microbial Culture Medium (NSM).

Gooseberry are plagued by a number of bacterial and fungi pathogens including Mucor sps, Rhizopus sps, Fusarium sps, Trichoderma sps and Klebsiella pneumonia, Staphylococcus aureus, Streptococcus pneumonia, Shigella sps ,and proteus. We developed a semi solid media, termed natural source medium (NSM), to selectively and rapidly isolate fungi and bacteria pathogenic to and associated with gooseberry and some other fruits. Most strains of interest grow sufficiently on NSM in 24h at 37⁰C for bacteria and 48h at room temperature for fungi tentative identification based on colony morphology, Gram staining and Biochemical characteristic.