Some biological characteristics of China strain of anopheles dirus in laboratory

The eggs of Anopheles dirus originated from China and currently colonized at the National Institute of Malariology, Parasitology and Entomology kept at 24 - 26°C on wet cotton for 20 - 25 days gave hatching rate approximately of 40%. The suitable food for Anopheles dirus larvae consisted of 6g bread powder, 2g green bean powder and 0.5mg vitamin B 1. The appropriate density for rearing An. dirus larvae is 0.4 larva per square centimetre of water surface

Preliminary dat on the colonizing of An.sundaicus in the laboratory

Freshly fed or half gravid An.sundaicus collected in An Thoi Dong commune, Can Gio district, Ho Chi Minh city were brought to the insectary of NIMPE for colonizing. Larvae were fed by a combination of 4gr bread powder + 4 shrimp powder + 2gr bean powder + 0.001gr vitamine B1. Larvae were contained at the conditions: a density of 0.3 larvae/cm2 of the surface water. Water for rearing was taken form the field with the salinity of 0.6 – 0.8%. Room temperature of 27 – 31oC, humidity of 60 – 90%. At these conditions, the image period took the time of 5 – 17 days. From the 8th generation (F8), mosquitoes were found to take free matting in the cage of 30 x 30 x 30 cm

Establishment of a stenogamous colony of Anopheles minimus theobald, 1901 in the laboratory

An. minimus was collected from Hoa Son village, Luong Son district, Hoa Binh province in November 1997 and maintained in the laboratory of NIMPE by artificial mating techniques. Since 51st generation, a free mating colony in the small cages (with dimensions of 30 x 30 x 30cm) has been established by releasing 200 male and 200 female mosquitoes per cage. The releasing of additional 50 males of An. minimus to the cage has been found to stimulate the mating process in small cage

The effect of illumination power, size of holding cages and rearing density on the development and reproduction of anopheles minimus theobald, 1901

Experiments were carried out on free mating An. minimus strain in the laboratory. Under condition of illumination power at 200-220 lux the rates of larvae developed to pupae and to adults were 62.7% and 59.3%, respectively. The mean life span of the males was shorter than that of females. At optimum rearing density of one mosquito per 1.3 cm2 of a vertical side of holding cage the inseminated rate was 72% and mean number of eggs was 145 per female. The size of 30 x 30 x 30 cm of holding cages and illumination power at 200 lux seemed to be suitable for efficient mating of An. minimus

Adaptation of anopheles sundaicus larvae in insectary conditions

An.Sundaucus collected from Can Gio, Ho Chi Minh City are colonized at the insectary of the National Institute of Malariology, Parasitology and Entomology and found to quickly adapt to the laboratory conditions. Since the F3, larvae have been living in the artificial brackish water with NaCl 1%. In this condition, 88% of larvae developed to pupae and 83.5% to adults. Light time 10 hours per day with 250 - 270 lux was determined to be conducive for development of larvae

Effects of temperature and source of blood on development of Anopheles minimus in laboratory

Experiments were caried out on An.minimus F98 in laboratory conditions of 22+/- 1°C, 25+/- 1°C and 28 +/- 1°C, 70-80% RH. The time required for egg stage and egg hatching rate were negatively correlated with temperature. At 22 +/- 1°C, egg hatching rate was 84.1 %, the duration of the egg stage was 3-4 days. At 25+/-1°C and 28 +/-1oC, egg hatching rate was 77.3% and 65.5%, respectively, the time of the egg stage were 2-3 days. At higher temperature, the duration of larvae stage was shorter than at the lower temperature. 25°C was optimum temperature for larvae with 38% of larvae developed to adults. 1 day after molting, mosquitoes started feeding at all tested temperatures. At 22°C, blood feeding rate higher than at the higher temperatures. Number of eggs and egg hatching rate of mosquitoes that fed human blood were higher than mosquitoes fed on mice