RESUMEN
@#Abstract: Methods - ( ) To investigate the repairing effect of adipose derived stem cells ADSCs transplantation in - - Methods 1 bromopropane induced peripheral nerve injury in rats. A total of 45 specific pathogen free male SD rats were ( ) ( ) randomly assigned to the control group 15 rats and exposure group 30 rats . The rats in the exposure group were gavaged with - , , 1 bromopropane at a dose of 800 mg/kg body weight while the control group were given with equal volume of corn oil once a , - day five days per week for eight weeks. The rat model of peripheral nerve injury induced by 1 bromopropane was established - , - and was randomly assigned to the self recovery group and ADSCs group 12 rats in each group. ADSCs group and self recovery ( 6 ) , group were injected with 0.5 mL contains 1×10 cells ADSCs or 0.5 mL phosphate buffer solution respectively by tail vein once a week for four weeks. The control group was not administered any treatment. The rats in each group were assessed using - - ( ) , , inclined plane test and Basso Beattie Bresnahan BBB score on the first day before treatment as well as 7 14 21 and 28 day , after treatment. At the end of treatment the sciatic nerve was isolated for histopathological examination. The oxidative stress - indexes in the cerebral motor cortex were detected by colorimetric analysis. The levels of brain derived neurotrophic factor ( ) ( ) - Results BDNF and nerve growth factor NGF in the sciatic nerve were detected by enzyme linked immunosorbent assay. The - maximum tilt angle of the inclined plate and the BBB score were lower in self recovery group at the five time points of treatment ( P< ) all 0.05 compared with the control group. The maximum tilt angle of the inclined plate test was higher in ADSCs group at 21 ( P< ), , , ( P< ), and 28 days of treatment all 0.05 and the BBB score was higher at 7 14 21 and 28 days of treatment all 0.05 - ( ) when compared with the self recovery group. The level of malondialdehyde MDA in the cerebral motor cortex increased (P< ), ( ) ( ) ( P< ), 0.05 while the superoxide dismutase SOD activity and glutathione GSH level decreased all 0.05 and the levels ( P< ) - of BDNF and NGF in the sciatic nerve decreased all 0.05 in self recovery group compared with the control group. There was , , no significant difference in SOD activity and MDA GSH BDNF and NGF levels between ADSCs group and control group ( P> ) (P< ), ( all 0.05 . The level of MDA in cerebral motor cortex decreased 0.05 the SOD activity and GSH level increased all P< ), (P< ) - 0.05 and the level of BDNF in sciatic nerve increased 0.05 in ADSCs group compared with the self recovery group. Conclusion - - ADSCs transplantation can repair peripheral nerve injury induced by 1 bromopropane via anti oxidative stress and regulating the secretion of neurotrophic factors.