Asunto(s)
Administración Tópica , Candidiasis/tratamiento farmacológico , Ácido Cítrico/administración & dosificación , Pie Diabético/tratamiento farmacológico , Quimioterapia Combinada , Violeta de Genciana/administración & dosificación , Humanos , Masculino , Persona de Mediana Edad , Infecciones por Pseudomonas/tratamiento farmacológico , Infecciones Estafilocócicas/tratamiento farmacológicoRESUMEN
A total of 114 opportunistic bacteria were isolated from 65 swabs from burn sepsis. P. aeruginosa (53.8%) was the most common agent followed by S. aureus (38.4%), Klebsiella Spp. (27.6%), Proteus (18.4%), E. coil (10.7%) and others. The infection was monobacterial in 25 cases (38.4%) and polybacterial in 49 cases (61.5%). P. aeruginosa was predominated in both monobacterial and polybacterial infections. Ciprofloxacin (42.9%) was found to be the most effective antibacterial agent. Results indicate that resistance in burn isolates is higher and increasing day by day.
Asunto(s)
Antibacterianos/farmacología , Bacteriemia/diagnóstico , Quemaduras/microbiología , Recuento de Colonia Microbiana , Femenino , Bacterias Gramnegativas/aislamiento & purificación , Bacterias Grampositivas/aislamiento & purificación , Humanos , Masculino , Pruebas de Sensibilidad Microbiana , Sensibilidad y Especificidad , Infección de Heridas/microbiologíaRESUMEN
It is a well established fact that dental caries is caused by streptococcus mutans. For qualitative and quantitative analysis of streptococcus mutans, saliva samples are often preferred and are processed according to Kohler and Brathall method. In the present study an attempt is made to relate caries status (caries free, average caries, and rampant caries group) with cfu counts of streptococcus mutans and Snyder test. The streptococcus mutans were isolated using MSB agar and cfu were determined of each individual from different caries groups. The saliva of the same individual was drooled into a culture bottle containing Snyder test agar and extent of colour change was observed after 24, 48, and 72 hours incubated at 37 degrees C. The time and extent of colour change determined the conduciveness of diet. It has been observed that caries free individuals have low cfu count and their diet was mild or moderately conducive, while the individuals having average caries had medium cfu count and their diet was moderately-high conducive. In rampant caries, individuals however the cfu count were high and the diet was highly conducive. In caries free, average caries and rampant caries group 40, 60, and 80 per cent samples showed colour change, in Snyder test up to full length in 72 hours respectively. Thus a definite correlation exists between caries status, cfu count of streptococcus mutans and Snyder caries activity test.
Asunto(s)
Niño , Preescolar , Recuento de Colonia Microbiana , Colorimetría , Estudios Transversales , Índice CPO , Caries Dental/microbiología , Pruebas de Actividad de Caries Dental , Susceptibilidad a Caries Dentarias , Dieta , Dieta Cariógena , Femenino , Humanos , Masculino , Saliva/microbiología , Streptococcus mutans/clasificación , Diente Primario/microbiología , Salud UrbanaRESUMEN
Two hundred and five specimens from various sources of hospital ecosystem were processed for isolation of bacteria. 51.7% specimens were found positive and a total of 146 isolates were obtained. Isolation was monobacterial in 35.6% and polybacterial in 16.0%. Bacteria were recovered from discarding jar fluid (84.6%), air (73%), basin swabs (70.5%) hand wash bowl solution (64.2%), cheatle forceps (64%) and thermometer fluid (53.8%) etc. The most common bacteria isolated were P. aeruginosa, B. subtilis, Klebsiella Spp. and E. col and ciprofloxacin (72.88%) was found to be the most effective antibacterial agent.
Asunto(s)
Microbiología del Aire , Bacterias/aislamiento & purificación , Farmacorresistencia Microbiana , Hospitales Rurales , India , Microbiología del AguaRESUMEN
Nine clinical isolates of coagulase negative staphylococci (CONS) susceptible to norfloxacin (MIC 1.8-2 micrograms/ml) were manipulated in vitro to induce norfloxacin resistance by means of serial passage in brain heart infusion broth containing increasing concentrations of norfloxacin. Exposure of CONS to norfloxacin resulted in 18 to 20 times increase in MIC of norfloxacin and change in in vitro susceptibility to ciprofloxacin, pefloxacin, ofloxacin, kanamycin, neomycin and tobramycin, indicating development of cross resistance to fluoroquinolones and aminoglycosides. These results show that exposure to increasing concentrations of norfloxacin can induce the development of resistance to various antimicrobial agents, suggesting its mutagenic role.
Asunto(s)
Antiinfecciosos/farmacología , Coagulasa/análisis , Farmacorresistencia Microbiana , Humanos , Norfloxacino/farmacología , Staphylococcus/efectos de los fármacosRESUMEN
Forty nine multiple drug resistant strains of E. coli isolated from UTI were serotyped. The pattern was found to be 057 (eight strains); 0109 (four strains); 020, 038, 068, 0106, 0148. Rough (three each). 012, 054, 0101, 0160 (two each) and 02, 032, 046, 053, 060, 065, 090, 091, 0117, 0118, untypable (one each). The resistance pattern of all E. coli were identified and matted with recepient strain in penassay broth and in human urine. In a penassay broth transfer of resistance was demonstrated in 38 strains (77.5%) while in human urine transfer was demonstrated only in 14 strains (28.57%).