RESUMEN
Hair evidence is among the most common types of physical evidence and may be found in a wide variety of cases. Hairs may be of particular interest in cases knows to involve personal contract, such as sexual assault, pedestrian motor vehicle accidents, and homicides. In addition, hair may also be an important piece of associative evidence to prove or disprove an event. The most common request that is made the laboratory when hair is used as forensic evidence is to determine whether or not hair recovered at the crime scene compares to hair removed from a suspect. In most cases, such a comparison relates to hair obtained from the scalp or public area. Ultimately, the evidential value of the comparison will depend on the degree of probability with which the examiner can associate the questioned hair to a particular individual The aim of the present study is to indentify individuals through their DNA makeup extracted from their hair, through the utilization of the polymerase chain reaction [PCR] to amplify the small amount of DNA found in the human hair root or in follicular tissue adhering to a pulled hair. The study was conducted on 60 randomly selected healthy adult males and females. Freshly plucked hair and blood samples were obtained from the subjects For the DNA extracted from hair quantitation was performed using the QuantiBlot Human DNA Quantitaiton kit. DNA extracted from root samples only was subjected to sex detrmination using GernePRINTrm. Sex Identification System- Amelogenin. Amplification for the DQA1 and PM loci was done using the Amplitype combiendf DQAl and PM PCR amplification and typing kit. The AmpliFLP DIS80 PCR Amplification kit was used for the amplification and analkysis of alleles at the DIS80 locus