P53 expression in glioma: an immunohistochemical study

The aim of this study was to evaluate the frequency of immunohistochemical [IHC] expression of p53 protein in different types of glioma in Mosul city, and to correlate p53 expression with the histological types and grades of gliomas, and compare the results of this study with those of others. This study was performed on 50 cases of glioma. Samples were obtained in a prospective and retrospective fashion [cross-sectional study]. The samples were collected during the period extending from October 2010 to May 2011. All cases were obtained from Al-Jamhuri Teaching Hospital in Mosul city, Northen Iraq and some private laboratories. Typing and grading of the glioma were done according to World Health Organization [WHO] classification system. P53 expression was assessed immunohistochemically. Fifty cases of gliomas were collected; they included 37 cases of astrocytoms, 8 ependymoma 4 oligodendrogliomas and 1 oligoastrocytoma. p53 expression was detected in 25 cases ofglioma [50%]. The positive cases included 59.45% ofastrocytomas, 50% of oligodendrogliomas, and one case of oligoastrocytoma which was positive also. On the other hand, all of the ependymomas were negative for p53 protein. p53 was significantly related to the grades of glioma, but not significantly related to the type ofglioma. P53 expression was expressed in 50% of gliomas in Mosul city. P53 expression is common among high grade astrocytomas and mixed gliomas, less among oligodendrogliomas, and lacking in cases of ependymomas. Statistically, p53 expression was not significantly correlated with the type of glioma. p53 index is directly correlated with the grade of glioma, and so it is of prognostic value

Ki-67[MIB-1] and progesterone receptor in meningioma: an immunohistochemical study

In meningioma the tumor grade, subtype, and extent of surgical resection are strong prognostic factors, the growth of this tumor is still unpredictable, and additional prognostic markers are needed. Many studies have shown that the detection of the proliferative potential of meningioma by Ki-67 [MIB-l] and the detection of the progesterone receptor might predict the natural history of tumor and patient survival. To evaluate the proliferative fraction Ki-67 [MIB-l] and progesterone receptor in meningioma, To correlate the expression of these two markers with various clinico- pathological parameters, To compare these results with other studies. This is a prospective study, in which 50 cases of meningioma were collected in Nineveh province. These cases were examined immunohistochemically. The results of these two markers were correlated with various clinico- pathological parameters. The mean age of all cases was 46 years. There were 16 male and 34 female patients. According to the W.H.O 2000 classification, there were 84% grade l, 10% were grade II and 6% were grade III. Only 14% of the patients had a history of recurrence. Progesterone receptor was positive in 72% of all cases. Significant relation was observed between the progesterone receptor and the patients age .It was significantly higher in female than male. A significant relationship was found between progesterone receptor with grade and histological types. Thirty three out of 43 cases without recurrence had positive progesterone receptor. The mean +/- Standard deviation [SD] of Ki-67 Labeling Index [Ki-67 LI] was 2.7 +/- 4.2%. No significant relation was found between the Ki-67 LI and patients age. The male patients had a higher Ki-67 Ll than that of the female. Significant relation was found between Ki-67 Ll and the grade of meningioma. The mean of Ki-67 LI in the recurrent cases was higher than in non recurrent cases. An inverse relationship was found between Ki-67 LI and progesterone receptor. Progesterone receptor showed a significant relation with the age and histological types. Ki-67 LI had a direct significant association with the grade and the recurrent cases, while inverse relation was observed between the progesterone receptor with the grade and the recurrent cases. An inverse relationship was observed between the progesterone receptor and Ki-67 LI

P53 expression in endometrial carcinoma an immunohistochemical study

The current study aims to evaluate the immunohistochemical expression of p53 protein in patients with endometrial carcinoma in correlation with other parameters including estrogen receptor, and with results of other studies. Sixty cases of endometrial carcinoma in which the estrogen receptor status was previously tested by immunohistochemical staining, were included in this study. The endometrial carcinoma was detected in 15 endometrial curetting and 45 hysterectomy specimens. The blocks of these cases were collected from Al Jumhuri Teaching hospital, AL-Khansaa and Al-Butool Teaching hospitals and private laboratories in Mosul city, during the period from January 2007 to January 2009. Tumors were classified according to the World Health Organization classification system and graded according to FIGO grading system. The expression of p53 protein was evaluated immunohistochemically. P53 expression was detected in 21 cases of endometrial carcinoma [35%]. The patients ages ranged from 40 to 70 years [mean=56.5 years], p53 expression has a statistically no significant relationship with patients' age. Fifty five cases [91.7%] were endometrioid carcinoma [type 1] and 5 cases [8.3%] were non-endometrioid carcinoma [type 2]. A statistically significant association was found between p53 expression and types of endometrial carcinoma [EC]. P53 expression was directly correlated with the grade of endometriod carcinoma and the extent of myometrial invasion. Positive expression of p53 was inversly significantly associated with positive estrogen receptor [ER] cases. About 75% of ER positive cases of EC [60%] failed to show p53 expression positivity. P53 expression was found in 35% of endometrial carcinoma in Mosul city. A significant association was found between p53 expression and tumor type. P53 expression is directly correlated with the age of the patients, grade of endometrioid carcinoma and the extent of myometrial invasion. On the other hand, there was a significant inverse relationship between p53 expression and estrogen receptor status