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Egyptian Journal of Medical Human Genetics [The]. 2017; 18 (2): 173-179
en Inglés | IMEMR | ID: emr-188479

RESUMEN

Background: MicroRNAs [miRNAs] are small non-coding RNAs, whose role in regulating diverse immune functions, suggests they might play a role as biomarkers for immune medi-ated disorders. Studies showed that miRNA-146a [miR-146a] expression is increased by proinflammatory cytokines and is an important modulator of differentiation and function of cells of innate and adaptive immunity


Aim of the work: The current study aimed to evaluate the expression of miR-146a as a potential biomarker for diagnosis of rheumatoid arthritis [RA] and to explore its association with disease activity


Subjects and methods: The study enrolled 50 Egyptian subjects divided into a patient group, which comprised 25 RA patients, and a control group which comprised 25 healthy individuals


The disease activity for the patients' group was determined by simplified disease activity index. Relative quantification of miR-146a expression in whole blood was determined using reverse tran-scriptase quantitative real time polymerase chain reaction


Results: There were highly significant statistical differences between patients and healthy controls as regards miR-146a relative expression, erythrocyte sedimentation rate [ESR] and anti-cyclic citrullinated peptide [anti-CCP] [p < 0.001]. Highly significant statistical differences [p < 0.001] were also found between different patients' subgroups as regards miR-146a relative expression and ESR. rm'R-146a levels correlated positively with those of ESR, C-reactive protein and anti-CCP [p < 0.001]


miR-146a illustrated best performance in diagnosing RA, showing the highest sensitivity and specificity [96% and 100%, respectively] [AUC: 0.992 at a cut off value of >/=2.16] compared to anti-CCP [sensitivity: 68%, specificity: 100% and AUC: 0.87 at a cut off value of >/= 22 U/ml] and RF [sensitivity: 56%, specificity: 80% and AUC: 0.992 at a cut off value of >/=13 U/ml]


Conclusion: This study demonstrated that miR-146a expression was highly significantly elevated in whole blood of patients with RA. Its diagnostic performance was better than anti-CCP and RF and its level of expression correlates with disease activity


Asunto(s)
Humanos , Femenino , Masculino , Adulto , Persona de Mediana Edad , MicroARNs , Expresión Génica , Anticuerpos Antiproteína Citrulinada , Factor Reumatoide , Biomarcadores , Estudios de Casos y Controles
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